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Chapter 1<br />

become colonized with >1000 CFU/ 5cm 2 <strong>of</strong> Salmonella [29]. Moore et al.<br />

also demonstrated that once a surface is colonized, Salmonella can readily<br />

transfer onto food products [30]. Subsequently contamination can result in<br />

food-borne infections if food is undercooked or incorrectly preserved<br />

(smoked, salted). Previous research has also shown that during handling<br />

and preparation <strong>of</strong> raw chicken (contaminated with Salmonella) that<br />

domestic kitchen surfaces, including chopping boards, utensils, taps and<br />

sink rims, fridge and oven doors can become colonised with the bacterium,<br />

which can still be present after cleaning with disinfectants [31]. It is<br />

estimated that cross contamination (from carcasses to surfaces and vica<br />

versa) in the slaughter process can account for 29% <strong>of</strong> the total <strong>of</strong><br />

Salmonella contaminated carcasses [32].<br />

The presence <strong>of</strong> bacterial cells persisting after cleaning may also lead to the<br />

development <strong>of</strong> a bi<strong>of</strong>ilm on the implicated surfaces and result in potential<br />

for recurring contamination events. Numerous food-borne outbreaks <strong>of</strong><br />

Salmonella have been linked to contaminated processing areas including a<br />

processing line [33-34], baking utensils [35] and in food storage containers<br />

[36].<br />

1.11. Salmonellosis in <strong>Ireland</strong><br />

The Computerized Infectious Disease Reporting system (CIDR) was<br />

established in 2004 to facilitate the collection <strong>of</strong> data relating to infectious<br />

diseases in <strong>Ireland</strong> [37]. The number <strong>of</strong> cases <strong>of</strong> Salmonella submitted to<br />

the <strong>National</strong> Salmonella, Shigella and Listeria Reference Laboratory<br />

(NSSLRL) for typing have declined by over 10% in the past 6 years. The two<br />

predominant serovars associated with human salmonellosis reported in<br />

<strong>Ireland</strong> were S. Enteritidis (18-41%) and S. Typhimurium (23-31%)[38] . This<br />

trend is also similar to other European countries [39]. S. Enteritidis was the<br />

Page 9

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