View/Open - ARAN - National University of Ireland, Galway

Chapter 5
5.6. Summary
S. enterica can form a biofilm using the microtitre plate method. Despite
the limitations of reproducibility using the microtitre plate method there
was some evidence to suggest that the S. Agona strains may form a more
dense biofilm that strains of S. Typhimurium and S. Enteritidis, particularly
at 22°C after 48 hours incubation. There is also some suggestion that in
most cases the S. enterica strains examined over 168 hours did not form a
more dense biofilm than the strain formed in 48 hours when incubated at
room temperature (22°C) but did at 37°C. Therefore an increase in
temperature may result in the density of biofilm formed on surfaces
increasing.
Furthermore the results presented in this chapter indicate that S. enterica
cells contained within a biofilm can remain viable after contact with three
disinfectant agents commonly used in industry, despite the use of
extensive contact times of up to 90 minutes. This may have seriously
implication on both domestic and food processing facilities if a biofilm
develops on the surfaces in contact with food.
Page 192