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Chapter 5
Table 5.2: The optical density of biofilm formed using the microtitre plate
Difference
Strain Well 1 st OD 590 Read 2 nd OD 590 Read ( 1 st -2 nd OD)
S. Agona S08-0601 1 0.344 0.218 0.126
2 1.641 0.272 1.369
3 0.141 0.100 0.041
S. Agona S09-0494 1 0.339 0.504 -0.165
2 0.260 0.245 0.015
3 0.130 0.113 0.017
S. Agona S09-0046 1 0.157 0.179 -0.022
2 0.091 0.107 -0.016
3 0.057 0.074 -0.017
S. Agona S09-0371 1 0.870 0.603 0.267
2 1.119 1.112 0.007
3 0.142 0.115 0.027
S. Agona S09-0479 1 0.119 0.109 0.010
2 0.074 0.072 0.002
3 0.209 0.168 0.041
S. Agona SL483 1 0.473 0.634 -0.161
2 0.589 0.566 0.023
3 0.819 0.571 0.248
Table 5.2 displays the optical density for Salmonella enterica subspecies
enterica serovar Agona biofilm formed in 3 replicate wells for each of the 6
S. Agona strains examined. The optical density was measured at 590
nanometres wavelengths (OD 590 ). The measurement of OD 590 was repeated
on the same plate twice to examine the repeatability of measurement of
OD 590 values. Each strain was examined in triplicate wells in the microtitre
plate. The values for the S. Agona strains are displayed in Table 5.2
however the values for all 12 S. enterica and the 1 Pseudomonas
aeruginosa strain when the OD 590 for each was measured twice in one
plate are listed in Table 1 in Appendix 2.
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