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Chapter 5
Figure 5.2: Stained biofilm attached to surface (48 hours at 22°C)
Figure 5.3: Stained biofilm attached to surface (48 hours at 37°C)
Figure 5.4: Stained biofilm attached to the surface (168 hours at 37°C)
Figures 5.2, 5.3 and 5.4 displays the crystal violet stain remained attached
to the surface of the microtitre plate post resolublising the stained biomass
with ethanol: acetone. The optical density was read with the ethanol:
acetone solution in the wells after which the solution was extracted to
examine the extent of crystal violet that failed to resuspend in the solution.
The amount of crystal violet attached to the material in the microtitre plate
is indicative of dye that failed to resuspend. The incomplete resuspension
of the stained biofilm may have contributed to large variation in repeated
measurements of optical density.
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