View/Open - ARAN - National University of Ireland, Galway

Chapter 4
provide a more standardised approach to examining the efficacy of
disinfectants against an established biofilm using the CBR (E2871-12) and
MBEC (E2799-12) biofilm development methods. However sonication
conditions used with the CBR method (are 3 cycles of sonication for 30
seconds with intermittent vortexing) are unlikely to achieve complete
removal of all biofilm based on data presented here. Other parameters
such as test organism, temperature, pH, and nutrient flow and organic
content should also be considered. Moreover, as the publication of these
standards is relatively recent, there is relatively little experience with their
use and it is not clear how widely they will be adopted due to the high
costs associated with the use of the equipment. Other factors such as the
method used to establish a biofilm may also influence the efficacy of test
products against microbial biofilms of the same strains [117, 134].
Page 149