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View/Open - ARAN - National University of Ireland, Galway

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Appendix 2<br />

Colour index<br />

High outlier<br />

Low outlier<br />

Large Difference in OD<br />

Appendix 2 - Table 1: displays the optical density bi<strong>of</strong>ilm formed in 3<br />

replicate wells for each for 6 Salmonella enterica subspecies enterica<br />

serovar Agona strains, 6 Salmonella Typhimurium strains and 2 Salmonella<br />

Enteritidis strains examined. The optical density was measured at 590<br />

wavelengths (OD 590 ). The measurement <strong>of</strong> OD 590 was repeated on the<br />

same plate twice to examine the repeatability <strong>of</strong> measurement <strong>of</strong> OD 590<br />

values. Each strain was examined in triplicate wells in the microtitre plate.<br />

The high outliers are denoted by yellow coloured cell and the low outliers<br />

are denoted by blue coloured cell. The difference between the repeated<br />

reads was calculated by subtracting the 1 st read from the 2 nd read <strong>of</strong> OD 590.<br />

A large difference in the reads is denoted by a pink coloured cell. The mean<br />

<strong>of</strong> all 6 observations for each strain was also calculated. These mean<br />

calculations are based on the bi<strong>of</strong>ilm density formed over 48 hours at 22°C.<br />

Page 255

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