Coordinated regulation of gene expression by E ... - Jacobs University

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MATERIALS AND METHODS dNTPs (25 μmol) NTPs Agarose Roche Roche Roti ® garose from Carl Roth GmbH, Germany. 2.1.2 Antibiotics The following antibiotics were prepared as described by Sambrook et al. [Sambrook and Russell 2001]. The concentration of their stock solution and final working concentrations used are indicated below. Stock solution Working concentration Ampicillin 50 mg/ml in water 20 μg/ml Chloremphinicol 34 mg/ml in ethanol 25 μg/ml Tetracyclin 5 mg/ml in ethanol 10 μg/ml Kanamycin 10 mg/ml in water 10 μg/ml 2.1.3 Media The media were prepared as described by Sambrook et al. Composition to prepare one liter of media is given below. 2x YT medium: Tryptone Yeast extract NaCl 16 gm 10 gm 5 gm LB medium: Tryptone Yeast extract 10 gm 5 gm 19
MATERIALS AND METHODS NaCl 10 gm YT agar plates: Tryptone Yeast extract NaCl Agar 8 gm 5 gm 5 gm 15 gm 2.1.4 Buffers and stock solutions TE buffer (pH 8.0): 10mM Tris/HCl 1mM EDTA TAE buffer (50X): 242 gm of Tris base 57.1 ml of glacial acetic acid 100 ml of 0.5M EDTA (pH 8.0) TBE buffer (5X): 54 gm of Tris base 27.5 gm of boric acid 20 ml of 0.5M EDTA (pH 8.0) Killing buffer: 20 mM Tris-Cl (pH 7.5) 5 mM MgCl 2 20 mM Sodium azide 10X in vitro transcription buffer: 100 mM Tris-HCl (pH 8.0) 2 M NaCl 100 mM MgCl 2 10 mM DTT 20
Page 1 and 2: Coordinated regulation of gene expr
Page 3 and 4: Parts of this work has been publish
Page 5 and 6: ABSTRACT promoter region determines
Page 7 and 8: ACKNOWLEDGEMENTS first impression o
Page 9 and 10: TABLE OF CONTENTS 2.2.5 Preparation
Page 11 and 12: LIST OF TABLES List of tables Table
Page 13 and 14: LIST OF FIGURES List of Figures Fig
Page 15 and 16: ABBREVIATIONS Abbreviations ∆61
Page 17 and 18: ABBREVIATIONS TBE Tris TS tyrT35U t
Page 19 and 20: INTRODUCTION Salmonella: “In more
Page 21 and 22: INTRODUCTION Figure 1: Schematic il
Page 23 and 24: INTRODUCTION UP element -35 -10 Spa
Page 25 and 26: INTRODUCTION can also influence elo
Page 27 and 28: INTRODUCTION have more base-pairs t
Page 29 and 30: INTRODUCTION Figure 6: Graphical re
Page 31 and 32: INTRODUCTION shown that H-NS reache
Page 33 and 34: INTRODUCTION formed as a repercussi
Page 35: MATERIALS AND METHODS 2 Materials a
Page 39 and 40: MATERIALS AND METHODS LZ41 3 pyrF28
Page 41 and 42: MATERIALS AND METHODS 2.2 Methods 2
Page 43 and 44: MATERIALS AND METHODS given using a
Page 45 and 46: MATERIALS AND METHODS phenol: chlor
Page 47 and 48: MATERIALS AND METHODS Filtered (by
Page 49 and 50: MATERIALS AND METHODS free water (A
Page 51 and 52: RESULTS 3 Results Growth phase-depe
Page 53 and 54: RESULTS Neither the fis nor the hns
Page 55 and 56: RESULTS these identified genes (by
Page 57 and 58: RESULTS furthermore the colour of t
Page 59 and 60: RESULTS During the entire growth cy
Page 61 and 62: RESULTS transcripts of a growth pha
Page 63 and 64: RESULTS phosphorylation, are associ
Page 65 and 66: RESULTS Table 4: RT-PCR analysis of
Page 67 and 68: RESULTS Figure 16: Schematic repres
Page 69 and 70: RESULTS parC) which allows the supe
Page 71 and 72: RESULTS Figure 19: In vitro transcr
Page 73 and 74: RESULTS contrast the tyrTD promoter
Page 75 and 76: DISCUSSION 4 Discussion The idea of
Page 77 and 78: DISCUSSION In a study by Balke & Gr
Page 79 and 80: DISCUSSION cells maintain viability
Page 81 and 82: DISCUSSION writhe in the UAS by FIS
Page 83 and 84: DISCUSSION 4.3.2 Mechanism of trans
Page 85 and 86: CONCLUSION 5 Conclusion Regulation
Page 87 and 88:
REFERENCES Bordes, P., Conter, A.,
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REFERENCES Gruber, T.M., and Gross,
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REFERENCES molecular basis for sele
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REFERENCES Pan, C.Q., Finkel, S.E.,
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REFERENCES Schroder, O., and Wagner
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REFERENCES Willenbrock, H., and Uss
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APPENDIX I gene blattner ratio p-va
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APPENDIX I Supplementary table - VI
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APPENDIX I fabB b2323 0.6336 0.0043
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APPENDIX I ibpA b3687 2.4960 0.0181
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APPENDIX I pepP b2908 1.2182 0.0368
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APPENDIX I speG b1584 0.4151 0.0001
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APPENDIX I ycjX b1321 4.0841 0.0141
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APPENDIX I yhfY b3382 1.4063 0.0453
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APPENDIX I gene blattner ratio A/B
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Statement of sources Declaration I
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