Coordinated regulation of gene expression by E ... - Jacobs University

MATERIALS AND METHODS
1X in vitro transcription stop solution:
1 mM EDTA
50 mM sodium acetate
0.2% SDS
1X primer extension stop solution:
50% (v/v) glycerol
10 mM EDTA (pH 8.0)
0.25% (w/v) bromophenol blue
0.25% (w/v) xylene cyanol FF
Lysozyme:
10 mg/ml in 10 mM Tris-Cl (pH 8.0)
6X gel loading solution:
0.25% (w/v) bromophenol blue
0.25% (w/v) xylene cyanol FF
40% (w/v) sucrose in water
Norfloxacin:
20 mg/ml stock prepared in 0.5M sodium hydroxide solution.
2.1.5 Bacterial strains
Table 1: Bacterial strains used
Strain Genotype Reference
CSH50 1
ara ∆(lac pro) thi
Miller, JH (1972), cold
spring hrbr prs.
CSH50 ∆fis CSH50 fis::kan (rpsL) Koch et al., 1988
CSH50 ∆hns CSH50 hns-205::Tn10 P1 transduction of
CSH50 with hns-
205::Tn10 from
MC4100 2 hns-205::Tn10
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