Coordinated regulation of gene expression by E ... - Jacobs University

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ACKNOWLEDGEMENTS Acknowledgements I would like to express my appreciation, gratitude and thanks to the following people who have supported this work in many ways. I am indepted to Prof. Dr. Georgi Muskhelishvili not just for guiding my PhD work but for inspiring scientific temper in me, for being a mentor to whom I always looked up to during predicament and have very rarely been not satisfied by his suggestions and opinions to problems both scientific and general. Sincere thanks to Jacobs University Bremen (formerely International University Bremen) and Max Planck Institute for Terrestrial Microbiology at Marburg for giving me an opportunity to pursue my PhD work. I thank my doctoral examination committee, Prof. AndrewTravers, Prof. Albert Jeltsch and Prof. Sebastian Springer for support and motivation during my time at Jacobs University and for their thorough appraisal of this dissertation. I also take this opportunity to thank my family: my father, my mother, my sister Rohini, my borther-in-law Prakash, my nephew Varun, my niece Varsha, my brother Prasad, my sister-in-law Shvetha and my twin nieces Anagha and Anusha. The love showered by these people has always provided inspiration and motivation for me. A special thanks to my group members Marci, Michi, Sebastian, Nico and Claudia who made working in the lab an enjoyable experience. I will always cherish the time I spent with these intelligent people. And also thanks to Sissi for inducing and offering creative inputs on improving the appearance of my thesis and presentation beside being a good friend. Thanks also to all my friends in Marburg all of whom gave a very nice
ACKNOWLEDGEMENTS first impression of Germany, so much so that I feel Marburg as my home town in Germany. The time at the university was pleasant because of my friends all of whom I wish to thank. And friends from IGC, Sanjay, Arun, Abhishek (more popularly known as ‘Badiya’) and Nandu for organizing and participating in the weekend parties.
Page 1 and 2: Coordinated regulation of gene expr
Page 3 and 4: Parts of this work has been publish
Page 5: ABSTRACT promoter region determines
Page 9 and 10: TABLE OF CONTENTS 2.2.5 Preparation
Page 11 and 12: LIST OF TABLES List of tables Table
Page 13 and 14: LIST OF FIGURES List of Figures Fig
Page 15 and 16: ABBREVIATIONS Abbreviations ∆61
Page 17 and 18: ABBREVIATIONS TBE Tris TS tyrT35U t
Page 19 and 20: INTRODUCTION Salmonella: “In more
Page 21 and 22: INTRODUCTION Figure 1: Schematic il
Page 23 and 24: INTRODUCTION UP element -35 -10 Spa
Page 25 and 26: INTRODUCTION can also influence elo
Page 27 and 28: INTRODUCTION have more base-pairs t
Page 29 and 30: INTRODUCTION Figure 6: Graphical re
Page 31 and 32: INTRODUCTION shown that H-NS reache
Page 33 and 34: INTRODUCTION formed as a repercussi
Page 35 and 36: MATERIALS AND METHODS 2 Materials a
Page 37 and 38: MATERIALS AND METHODS NaCl 10 gm YT
Page 39 and 40: MATERIALS AND METHODS LZ41 3 pyrF28
Page 41 and 42: MATERIALS AND METHODS 2.2 Methods 2
Page 43 and 44: MATERIALS AND METHODS given using a
Page 45 and 46: MATERIALS AND METHODS phenol: chlor
Page 47 and 48: MATERIALS AND METHODS Filtered (by
Page 49 and 50: MATERIALS AND METHODS free water (A
Page 51 and 52: RESULTS 3 Results Growth phase-depe
Page 53 and 54: RESULTS Neither the fis nor the hns
Page 55 and 56: RESULTS these identified genes (by
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RESULTS furthermore the colour of t
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RESULTS During the entire growth cy
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RESULTS transcripts of a growth pha
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RESULTS phosphorylation, are associ
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RESULTS Table 4: RT-PCR analysis of
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RESULTS Figure 16: Schematic repres
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RESULTS parC) which allows the supe
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RESULTS Figure 19: In vitro transcr
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RESULTS contrast the tyrTD promoter
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DISCUSSION 4 Discussion The idea of
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DISCUSSION In a study by Balke & Gr
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DISCUSSION cells maintain viability
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DISCUSSION writhe in the UAS by FIS
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DISCUSSION 4.3.2 Mechanism of trans
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CONCLUSION 5 Conclusion Regulation
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REFERENCES Bordes, P., Conter, A.,
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REFERENCES Gruber, T.M., and Gross,
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REFERENCES molecular basis for sele
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REFERENCES Pan, C.Q., Finkel, S.E.,
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REFERENCES Schroder, O., and Wagner
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REFERENCES Willenbrock, H., and Uss
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APPENDIX I gene blattner ratio p-va
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APPENDIX I Supplementary table - VI
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APPENDIX I fabB b2323 0.6336 0.0043
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APPENDIX I ibpA b3687 2.4960 0.0181
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APPENDIX I pepP b2908 1.2182 0.0368
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APPENDIX I speG b1584 0.4151 0.0001
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APPENDIX I ycjX b1321 4.0841 0.0141
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APPENDIX I yhfY b3382 1.4063 0.0453
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APPENDIX I gene blattner ratio A/B
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Statement of sources Declaration I
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