CLINICAL LAB SCIENEC

CHAPTER 8: PIPETTING AND USE OF GLASSWARE 205
may measure amounts of sample as small as 2 to 10 μL. This type requires a
pipetter that may be adjustable to several volumes or may be fixed in the volume
aspirated. Accuracy levels for these sensitive pieces of equipment are remarkably
good. Piston-driven pipettes are found in technically advanced automated equipment
and require calibration and cleaning on a regular basis.
Review Questions
1. A 24-hour urine specimen is received in the laboratory. It is to be measured
to within a few milliliters of an exact volume. What type of laboratory container
would be best for measuring the volume of urine?
2. A volume of 200 mL of a 50:50 ratio of reagents A and B are to be mixed
to prepare a working solution for a certain laboratory procedure. Which
container would be most appropriate for preparing this reagent?
3. Describe the difference between a serological pipette and a Mohr pipette.
4. Why is it necessary to dispose of damaged glassware?
5. A patient’s sample requires the most accurate method of measuring due to
the extremely small volume used. Which pipette would be most useful for
this measurement?
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