CLINICAL LAB SCIENEC

370
ESSENTIALS OF CLINICAL LABORATORY SCIENCE
In the Lab
Equipment and Supplies
1. Gloves, disposable paper towels, and disinfectant or other cleaning solution
2. Report form provided by your instructor
3. Serum (preferred because fibrinogen is contained in a plasma sample)
a. For accuracy, the sample must be free of significant hemolysis.
b. A serum sample that is free from gross hemolysis or lipemia will remain
stable for up to one week at room temperature and for 30 days at 2°C–8°C.
c. When a blood sample is centrifuged immediately after clotting and the
serum is removed from the blood cells, hemolysis is greatly reduced.
4 Micropipetter with disposable pipette tips for measuring patient specimen as μLs
5. Serological pipettes
6. Stanbio kit for total protein (contains the reagent copper sulfate pentahydrate in
aqueous sodium hydroxide and aqueous protein standard [l0 mg/dL])
7. Spectrophotometer capable of absorbance readings at 550 nm (Figure 14-2)
8. Water bath or heat block set at 37°C (optional) (The reaction takes 10 minutes at
room temperature, and approximately 3 minutes at 37°C.)
9. Cuvettes
10. 13 × 100 test tubes and test tube rack
11. Waterproof sharp marker for labeling test tubes
12. Vortex mixer (optional) (desirable since the complex of the dye and proteins will
fall to the bottom of the tube in which the specimen and reagents are mixed and
even in the cuvettes before reading them on the spectrophotometer)
13. Electronic timing device or clock/watch with second hand
Procedure
1. Wash hands and don gloves.
2. Assemble necessary equipment and supplies.
A
B
FIGURE 14-2 (A) Spectronic Genesys 5-UV visible spectrophotometer, and (B) with printer.
Source: Delmar/Cengage Learning.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.