CLINICAL LAB SCIENEC

CHAPTER 17: IMMUNOHEMATOLOGY (BLOOD BANKING) 425
Equipment and Supplies
1. Gloves, disposable paper towels, and disinfectant
or other cleaning solution
2. Report form
3. EDTA anticoagulated blood sample or a
freshly clotted sample
4. Waterproof sharp marker
5. Normal physiological (0.85%) saline (blood
bank quality) in squeeze bottle
6. Disposable Pasteur pipettes or transfer
pipettes
FIGURE 17-2 ABO slide grouping procedures.
7. Anti-A blood serum
Source: Delmar/Cengage Learning.
8. Anti-B blood serum
9. Applicator sticks or plastic stirrers
10. Clean microscope slides or specialized cell typing slides
11. Illuminating lamp with magnifying mirror (optional)
12. Electronic timing device or clock/watch with second hand
13. Disposal containers appropriate for biohazardous waste and sharps container
Procedure
1. Wash hands and put on gloves.
2. Assemble equipment and materials.
3. Mark a clean slide into two halves, labeling the left side “A” and the right
side “B.”
4. Using a Pasteur pipette, transfer pipette, or dropper included with the reagent, place
one drop of anti-A serum on the “A” side (do not allow the dropper to touch slide).
5. Using a Pasteur pipette, transfer pipette, or dropper included with the reagent, place
one drop of anti-B serum on the “B” side (do not allow the dropper to touch slide).
8. Add one drop of well-mixed blood to each side of the slide using the Pasteur
pipette or a pair of applicator sticks (the drop of blood should be no larger than
the drop of antiserum).
9. Using a clean applicator stick, mix the blood and antiserum on side A and spread
into a smooth round circle about the size of a nickel.
10. Using a clean applicator stick, repeat the procedure on side B.
11. Rock the slide gently for 2 minutes and look for agglutination. An illuminating
lamp with magnifying mirror may be used as an optional practice.
12. Record which sides of the slide show agglutination, using the following form.
Place a plus sign (“+”) for agglutination into the appropriate blank(s) and a minus
sign (“–“) to indicate no agglutination.
Results
Side A
Side B
13. Discard all supplies used for the procedure in the appropriate containers.
A disinfectant should be used to clean the work surfaces and equipment
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