CLINICAL LAB SCIENEC

CHAPTER 13: HEMATOLOGY AND COAGULATION 339
18. Clean the microscope stage with lens paper or tissue. Clean the condenser if
necessary.
19. Retain the slide if required.
20. Discard all supplies used for the procedure in the appropriate containers. A disinfectant
should be used to clean the work surfaces and equipment should be
cleaned and restored to its former position. Gloves should be discarded appropriately
and the hands washed thoroughly in accordance with established policies.
Reporting of Results
Calculate the percentage of each of the five basic leukocytes (neutrophils, eosinophils,
basophils, lymphocytes, and monocytes). Report the direct leukocyte count
(DLC) as a percentage. If the total WBC is known, the count may also be reported in
absolute numbers by multiplying the percentages of each type by the actual WBC
count. Under “Comments”, note the presence of any immature cells, especially blast
cells (early immature types), and report these as percentages also.
In addition, the numbers and morphology of platelets can also be evaluated along
with the RBCs. Report your findings from the Practical White Blood Cell Differentiation
procedure in the Procedure #2 Report Form or other form supplied by your instructor.
Procedure #2 Report Form: Practical White Blood Cell (WBC) Differentiation
Patient Name: _____________________ Pt. ID _______________ Date: _____________
Age: _______ Gender (Circle One) M F Physician: ____________________________
Time Requested: ___________ Time Performed: ___________ Time Reported: __________
Examination Requested: WBC Differential Other
White Blood Cells
Neutrophils _________% Lymphocytes _________% Monocytes _________%
Eosinophils _________% Basophils _________% Bands _________%
Comments (unusual findings) _____________________________________________________
Performed by: ______________________ Date Reported _________________________
Evaluation of Student Results for Procedure #2
The differentials have been evaluated by counting 200 cells. Grades will be determined
as follows:
1. Segmented neutrophils and lymphocytes must come within ±2% of the target
value determined by the instructor. In the case that the total percentage is
extremely low (i.e., a lymphocyte count of less than 10%), two cells on either side
of the target value will be acceptable.
2. One letter grade will be deducted for EACH type of cell that is not within an
acceptable range.
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.