CLINICAL LAB SCIENEC

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ESSENTIALS OF CLINICAL LABORATORY SCIENCE
In the Lab
9. Serological pipettes
10. Electronic timing device or clock/watch with second hand
Procedure
1. Wash hands and don gloves.
2. Assemble necessary equipment and supplies.
3. Using a serological pipette, place into cuvettes the following volumes (mL) and
mix well. The volumes may be increased if the instrument requires volumes
greater than 1.0 mL.
Reagent Blank (RB) Standard (S) Controls (C) Unknowns (U)
Reagent 1.0 mL 1.0 mL 1.0 mL 1.0 mL
Standard - 10 μL - -
Controls - - 10 μL -
Unknowns - - - 10 μL
4. Incubate all cuvettes at 37°C for 10 minutes, or incubate at room temperature for
20 minutes.
5. Read S (standard) and RB (reagent blank) at 500 nm within 15 minutes.
6. Discard all supplies used for the procedure in the appropriate containers. A disinfectant
should be used to clean the work surfaces and equipment should be
cleaned and restored to its former position. Gloves should be discarded appropriately
and the hands washed thoroughly in accordance with established policies.
Quality Control
Two levels of control material, including both a normal and an abnormal value, with
known glucose levels, should be analyzed by this method on each day of testing.
Reporting of Results
Results are obtained using the following equation:
Calculation
Glucose (mg/dL) = Absorbance of unknown (Au) 100
Absorbance of standard (As)
where Au is the absorbance value of the unknown, As is the absorbance value of
the standard, and 100 is the concentration of the standard in milligrams per deciliter
(mg/dL).
Results are reported in milligrams per deciliter (mg/dL). In some countries, they are
reported in SI (International System) units that are derived from the metric system.
Conversion to SI units is often performed by the instrument upon which the test is
performed. Some health care facilities report values in both SI and metric units of
measurement.
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