CLINICAL LAB SCIENEC

CHAPTER 14: CLINICAL CHEMISTRY 375
12. Discard all supplies used for the procedure in the appropriate containers.
A disinfectant should be used to clean the work surfaces and equipment should
be cleaned and restored to its former position. Gloves should be discarded
appropriately and the hands washed thoroughly in accordance with established
policies.
Quality Control
Two to three levels of control material with known ranges of glucose levels determined
by this procedure should be analyzed each day of testing in a medical laboratory.
Linearity
When performed as directed this method provides for linearity from 0 to 500 g/dL.
Reporting of Results
Report your findings from the Glucose procedure in the form supplied by your instructor.
Results of serum or plasma glucose are reported in milligrams per deciliter (mg/dL).
The glucose determinations for some body fluids, including cerebrospinal fluid (CSF),
is always lower than the values found in the blood, but are performed in the same
manner as for serum or plasma. The value for CSF fluid is normally only about 60% – 70%
of that of the blood.
MANUAL CHEMISTRY PROCEDURE #3
Total Cholesterol and High-Density Lipoprotein (HDL)
Principles
The presence of excess lipids (of which cholesterol is but one type) in the blood has
been recognized as a risk factor for cardiovascular disease leading to stroke or heart
attack for years. A number of procedures are available for measuring cholesterol. In
the manual procedure presented here, total cholesterol is measured first. Low-density
lipoproteins (LDL) are then removed from the sample with a precipitating reagent,
leaving only the high-density lipoproteins (HDL), and a repeat procedure for cholesterol
is performed. The difference between the total cholesterol and the HDL value is
the LDL value.
The Stanbio Cholesterol and HDL Test Kits are intended for the quantitative, colorimetric
determination of cholesterol in serum or plasma. Standard laboratory tests for
measuring HDL are not usually performed in the clinical laboratory.
Equipment and Supplies
1. Gloves, disposable paper towels, and disinfectant or other cleaning solution
2. Report form provided by your instructor
3. Serum sample (must be free of hemolysis, separated from blood cells within
30 minutes of collection, and tested as soon as possible following collection)
4. Pipetter with disposable pipette tips for measuring 10 and 25 μLs
5. Serological pipettes
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