CLINICAL LAB SCIENEC

CHAPTER 12: PROCEDURES FOR URINALYSIS AND BODY FLUIDS 321
d. As with the undiluted specimen, count the WBCs and RBCs in all nine of the
large squares on both sides of the hemacytometer.
e. Average the two sides and multiply the results by 1.11.
f. Multiply the final result by 10 to account for the dilution.
6. Discard all supplies used for the procedure in the appropriate containers. A disinfectant
should be used to clean the work surfaces and equipment should be
cleaned and restored to its former position. Gloves should be discarded appropriately
and the hands washed thoroughly in accordance with established policies.
Reporting of Results
Report the cell count as cells/μL for both WBCs and RBCs.
URINALYSIS AND BODY FLUIDS ANALYSIS PROCEDURE #9
Determining Glucose in Cerebrospinal Fluid
Principles
CSF levels of protein and glucose are indicative of bacterial and viral infections,
based on which constituent is elevated or decreased. Both protein and glucose
levels in CSF are slightly lower than the levels found in the blood under normal
circumstances.
Abnormal results include increased and decreased glucose levels. Abnormal
results may be due to infection (bacterial or fungal), inflammation of the central nervous
system, or tumor.
The following procedure is adapted from the glucose kits available from Stanbio
Laboratory, Boerne, TX. Any reliable glucose procedure that employs either enzymatic
or end-point colorimetric methodology may be used for CSF glucose determinations.
Specimen Collection and Preparation
CSF glucose procedures may be performed on any of the 3 tubes normally collected
from a lumbar puncture. Centrifuge all specimens containing RBCs or particulate matter.
Equipment and Supplies
1. Gloves, disposable paper towels and disinfectant or other cleaning solution
2. Report form for recording results
3. CSF sample (Centrifuge to remove RBCs or particular matter and use the supernatant
for testing for glucose.)
4. Reagents for the quantitative enzymatic or colorimetric determination of glucose
in CSF. These are the same as those used for serum or plasma testing.
5. Glucose reagents and standards
6. Cuvettes for determining absorbance of protein or glucose
7. Spectrophotometer capable of reading turbidity or color, depending on methodology
chosen
8. Micropipetter
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