CLINICAL LAB SCIENEC

CHAPTER 13: HEMATOLOGY AND COAGULATION 357
9. Squeeze the reservoir gently two or three times to rinse the capillary pipette,
forcing diluent into but not out of the overflow chamber at the end of the pipette.
Release the pressure each time to allow adequate mixing of any diluents and
blood from the capillary pipette into the large chamber. Close the upper opening
with your index finger and invert the unit several times to mix the blood sample
and diluent as shown (Figure 13-18).
10. To prevent evaporation when storing a diluted specimen, cover the overflow
chamber of the capillary pipette with the capillary shield that was used to puncture
the diaphragm of the reservoir.
11. Just before beginning the cell count, again mix the blood and diluent by gently
inverting the reservoir several times while taking care to cover the hole with your
index finger.
A
B
C
FIGURE 13-18 (A) Inserting the capillary pipette filled with blood into the reservoir. (B) Releasing
pressure on reservoir to draw blood sample into diluents. (C) Insuring mixing of blood and diluents
in the reservoir.
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