CLINICAL LAB SCIENEC

CHAPTER 13: HEMATOLOGY AND COAGULATION 355
Correction Factor for Reticulocyte Count
A Corrected Reticulocyte Count may be performed to correct for anemia (low RBC
count), as shown in the following equation.
Patient’s reticulocyte count Patient’s Hct Corrected Reticulocyte Count
Normal Hct (45)
Example 13-2
Assume the patient’s hematocrit 34%. Using the numbers in Example 13-1:
1.2% 34 0.9% (corrected reticulocyte count)
45
Procedure #6 Report Form: Reticulocyte Count
Patient Name: _____________________ Pt. ID _____________________ Date: ________
Age: _______ Gender (Circle One) M F Physician: ____________________________
Time Requested: ___________ Time Performed: ___________ Time Reported: __________
Examination Requested: Reticulocyte Count Other
Procedure Results
Test Results Normal Range Correction Factor (Pt. HCT/45 X % Counted
Comments (unusual findings) _____________________________________________________
Performed by: _______________________ Date Reported _________________________
HEMATOLOGY AND COAGULATION PROCEDURE #7
Unopette Procedure for WBC Counts
Principles
This method uses a Unopette disposable diluting pipette system that provides
a convenient, precise, and accurate method for obtaining a WBC count from
blood and body fluids. When the Unopette method is used, whole blood is added to
a diluent. The diluent lyses (destroys) the RBCs but preserves and slightly stains the
WBCs. Once the red cells are completely lysed, the solution will be clear. The diluted
blood is then added to a hemacytometer. Once the hemacytometer is loaded, the
cells should be allowed to settle for 10 minutes before beginning the count.
The Unopette disposable diluting pipette system used to count WBCs is almost
identical in shape and application to the Unopette system for RBC counts. The only
major difference is that the reservoir contains a different diluent and the capillary
pipette capacity differs (RBC 10 μL and WBC 20 μL).
Equipment and Supplies
1. Gloves, disposable paper towels, and disinfectant or other cleaning solution
2. Report form for Procedure #7
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