CLINICAL LAB SCIENEC

310
ESSENTIALS OF CLINICAL LABORATORY SCIENCE
In the Lab
3. Place an Acetest tablet on a filter paper disc or other white absorbent paper.
4. Place 1 drop of urine directly on the Acetest tablet (Figure 12-35).
5. At 30 seconds, compare the color of the tablet with that on the chart provided by
the manufacturer.
6. Discard all supplies used for the procedure in the appropriate containers.
A disinfectant should be used to clean the work surfaces and equipment should
be cleaned and restored to its former position. Gloves should be discarded
appropriately and the hands washed thoroughly in accordance with established
policies.
Reporting of Results
Report on the Procedure Report Form or other appropriate form the semiquantitative
amounts of acetone present as follows:
Negative 1+ (small) 2+ (moderate) 3+ (large)
URINALYSIS AND BODY FLUIDS ANALYSIS PROCEDURE #4
Ictotest—Semiquantitative Test for Bilirubin in Urine
Principles
Bilirubin is a product derived from the breakdown of red blood cells. Extremely high
levels of bilirubin may be found in liver disease, where the liver is unable to form other
products from the excess hemoglobin, and in cases of high destruction of red blood
cells. Testing for the presence of abnormal levels of bilirubin in the urine is most often
performed by using Ictotest reagent tablets. When exposed to clinically significant levels
of bilirubin in the urine, these tablets react based on the coupling of a unique solid
diazonium salt with bilirubin in an acid medium, resulting in a blue or purple reaction
product. Mild colors, including pink, are not to be reported as positive bilirubin findings.
Pyridium is a medication sometimes used in cystitis (bladder infections) that will
mask positive results when small amounts of bilirubin are present. Other drugs (e.g.,
chlorpromazine or etodolac) may also cause a false positive result or atypical results.
FIGURE 12-35 Acetest for the presence of urinary ketones.
Source: Delmar/Cengage Learning.
Equipment and Supplies
1. Gloves, disposable paper towels and disinfectant
or other cleaning solution
2. Report form for urinalysis results (Procedure
Report Form)
3. Fresh urine sample that has tested positive
for bilirubin by dipstick (see
Procedure #1)
4. Disposable droppers
5. Deionized or distilled water
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