CLINICAL LAB SCIENEC

CHAPTER 17: IMMUNOHEMATOLOGY (BLOOD BANKING) 429
6. Disposable Pasteur pipettes or transfer pipettes
7. Anti-D blood serum
8. Rh control (negative control) (available commercially)
9. Positive whole blood Rh control (optional)
10. Clean microscope slides
11. Lighted and heated view box
12. Applicator sticks or plastic stirrers
13. Disposable 12 × 75 mm test tubes and test tube rack
14. Heat block set at 37°C
15. Centrifuge (serofuge) with head that will contain 12 × 75 mm tubes at 2000 rpm
16. Illuminating lamp with magnifying mirror (optional)
17. Disposal containers appropriate for biohazardous waste and sharps container
Procedure—Slide Test for Rh Factor
1. Wash hands and don gloves.
2. Assemble equipment and materials.
3. Mark a clean slide into two halves using a wax pencil, labeling the left side “A”
and the right side “control.”
4. Place a drop of the EDTA-anticoagulated blood on the test section of the slide
and one drop of the negative control on
the other section.
5. Place one drop of anti-Rh antisera from a
patient who is Rh negative and has developed
a high level (titer) of antibodies to
the Rh factor (D) on both the test section
and the control section of the slide.
6. Place the slides on the warming view box.
7. Mix each side with a clean applicator
stick and tilt the slide by rocking the
warming view box, ensuring the slide
does not move about on the glass surface,
by anchoring it with a fingertip.
8. Agglutination of the test drop indicates the
presence of the D antigen (Figure 17-4).
The control should be negative. If it is not,
there is a problem with the test reagents
or performance, and the test should be
repeated after appropriate modifications.
FIGURE 17-4 A heated and lighted viewbox is designed for Rh slide
typing, as the test requires heat for reaction. The specimen on the
right is positive for “big D” or the Rh factor.
Source: Delmar/Cengage Learning.
9. Further interpretation is required with apparently negative results with a modified
tube test for confirmation for the weak D (D u ).
10. Discard all supplies used for the procedure in the appropriate containers.
A disinfectant should be used to clean the work surfaces and equipment should
be cleaned and restored to its former position. Gloves should be discarded
appropriately and the hands washed thoroughly in accordance with established
policies.
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