CLINICAL LAB SCIENEC

362
ESSENTIALS OF CLINICAL LABORATORY SCIENCE
In the Lab
Procedure #8: Manual Prothrombin Time (PT)
Patient Name: _____________________ Pt. ID _____________________ Date: ________
Age: _______ Gender (Circle One) M F Physician: ____________________________
Time Requested: ___________ Time Performed: ___________ Time Reported: __________
Examination Requested: Manual Prothrombin Time Other
Tube #1 Tube #2 Average (Tube #1 Tube #2) / 2 INR Results
INR Calculation
INR [Patient Result in seconds / Mean normal of the facility in seconds] ISI*
*ISI supplied by manufacturer of reagents
Comments (unusual findings) _____________________________________________________
Performed by: _______________________ Date Reported _________________________
Summary
Hematology, the study of blood, is usually incorporated with basic tests for coagulation
(hemostasis). Most disease processes will show some sort of changes in the blood
picture, depending on the medical condition of the individual being tested. Samples
for testing require care in proper collection, processing, and sometimes storage.
A complete blood count (CBC) is the most common set of procedures performed
in hematology, but hundreds of specialized tests are also performed in
larger laboratories. This chapter referred to a number of manual tests that combine
to comprise a CBC, as well as two of the more common coagulation tests.
The clinical laboratory student can learn the basics of hematology and coagulation
by performing the manual procedures that make up a CBC, as well as the
calculations to provide a comprehensive blood picture.
A great deal of diagnostic information can be obtained from the proper collection
of a blood sample, the proper preparation of blood films, and careful attention to the
procedures for each analysis. In addition to blood counts, the performance of bleeding
tests and PTs will greatly prepare the student for a satisfactory clinical experience.
Review Questions
1. What values are contained in the RBC indices?
2. Why is it essential to have a properly prepared blood smear that is adequately
stained?
3. Why is it necessary to increase the amount of time required for blood to clot?
4. How does the WBC count along with a differential count help to diagnose
infection?
5. What is the importance of the INR when performing PT measurements?
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