CLINICAL LAB SCIENEC

CHAPTER 12: PROCEDURES FOR URINALYSIS AND BODY FLUIDS 313
5. Drop 1 Clinitest tablet into the test tube containing
the water and urine (or fecal) sample.
When a fecal sample is used, refer to
the collection procedures described under
the principles section for this procedure.
a. Watch for a boiling reaction.
b. Do not shake the tube during the reaction
or for 15 seconds after the boiling
stops.
c. Remember to watch for what is called
the “pass-through phenomenon.” This
action is defined as a series of color
reactions over a period of time until the
end-point occurs.
6. Determine results of the procedure from the
chart provided with the manufacturer’s kit.
7. Discard all supplies used for the procedure
in the appropriate containers. A disinfectant
should be used to clean the work
surfaces and equipment should be cleaned
and restored to its former position. Gloves
should be discarded appropriately and the hands washed thoroughly in accordance
with established policies.
Reporting of Results
Record the semiquantitative results on the Procedure Report Form or other appropriate
form as follows:
0 Trace ½% 1% 2% 3% 5%
0 trace 1 /2% 1% 2% 3% 5%
FIGURE 12-37 Performing Clinitest by placing 5 drops of urine
into test tube.
Source: Delmar/Cengage Learning.
URINALYSIS AND BODY FLUIDS ANALYSIS PROCEDURE #6
Confirmatory Semiquantitative Test for Total Protein in Urine
Principles
Measurement of urinary proteins is becoming increasingly important in the detection
of renal pathology. Proteinuria (increased amounts of protein in urine) can occur
in increased glomerular permeability, defective tubular reabsorption, and
abnormal secretion of protein into the urinary tract. Proteinuria can also be transient,
during cases of urinary tract infection or severe stress. Albuminuria (increased
albumin in urine) has been implicated as an early indicator of renal damage in diabetes
that can be reversed if identified and treated sufficiently early. Measurement
of total protein and specific proteins in cerebrospinal fl uid (CSF) is used to detect
increased permeability of the blood–brain barrier.
Often it is necessary to measure components of urine that may be positive when
screened by a dipstick, as certain conditions may yield a false positive. These confirmatory
tests can be time consuming but should be performed to avoid unnecessary
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