CLINICAL LAB SCIENEC

CHAPTER 12: PROCEDURES FOR URINALYSIS AND BODY FLUIDS 311
6. Absorbent square pads (test mats) upon
which the tablet will be placed and the
reaction will occur (provided with the kit
containing tablets)
7. Stoppered dark container of Ictotest tablets
(examine for color changes or
deterioration)
8. Electronic timing device or clock/watch
with second hand
Procedure
1. Wash hands and don gloves.
2. Assemble necessary equipment and
supplies.
3. Place a square of the absorbent test mat
onto a sheet of white paper.
4. Place 10 drops of urine on the center of the test mat.
5. Place 1 Ictotest tablet on the moistened mat.
Weak positive
Strong positive
FIGURE 12-36 Ictotest for bilirubin, performed on test mat.
Source: Delmar/Cengage Learning.
6. Carefully place 1 drop of deionized or distilled water on the top of the tablet (Figure
12-36). Wait 5 seconds. Add a second drop of water to the tablet so that the water
runs off the tablet and onto the mat.
7. The presence of a blue or purple color on the mat indicates a positive test for bilirubin.
A slight pink or red color should be ignored.
8. The test is subjectively graded as either negative or positive. See manufacturer’s
product insert with images of positive results.
9. Discard all supplies used for the procedure in the appropriate containers. A
disinfectant should be used to clean the work surfaces and equipment should
be cleaned and restored to its former position. Gloves should be discarded
appropriately and the hands washed thoroughly in accordance with established
policies.
Reporting of Results
Report on the Procedure Report Form or other appropriate form the amounts of bilirubin
present as either positive or negative only. There is no semiquantitative determination
for this procedure.
URINALYSIS AND BODY FLUIDS ANALYSIS PROCEDURE #5
Clinitest—Semiquantitative Test for Glucose and Other Reducing Substances
Principles
The presence of a number of reducing substances may be determined by the Clinitest
method even when a negative result has been found with the use of dipsticks, as
most dipstick brands are specific for glucose by using the glucohexokinase method.
The Clinitest will provide a positive result for any of the reducing substances,
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