CLINICAL LAB SCIENEC

372
ESSENTIALS OF CLINICAL LABORATORY SCIENCE
In the Lab
Quality Control
Two to three levels of control material with known ranges of protein levels determined
by this procedure are analyzed each day of testing in a medical laboratory. The total
protein value of each specimen can be estimated by using a refractometer where the
specific gravity is determined on the appropriate scale (a separate scale is observable
for urine samples).
Linearity
When performed as directed this method provides for linearity from 0 to 10 g/dL.
Reporting of Results
Report your findings from the Total Protein procedure in the form supplied by your
instructor. Results of the serum total protein are measured in grams per deciliter (g/dL).
However, the protein values for some body fluids, including cerebrospinal fluid and
urine, are measured in mg/dL.
MANUAL CHEMISTRY PROCEDURE #2
Glucose
Principles
Glucose is the major carbohydrate found in the blood and is used for energy for the
organs and tissues of the body. The glucose determination is chiefly used to determine
if a person is diabetic or, if the patient has already been diagnosed as diabetic, if
he or she is compliant in taking insulin and if the dosage is correct. The test may also
determine if a patient is hypoglycemic, a condition in which a low blood glucose is
found.
The Stanbio Enzymatic Glucose procedure is specific for glucose and may be used
for manual as well as automated systems. It uses an enzymatic procedure for the
quantitative enzymatic-colorimetric determination of glucose in serum, plasma, or
cerebrospinal fluid.
The reagent for this reaction provides for an enzymatic determination, and is the
most common method for determining glucose values. Reagents including enzymatic
glucose reagent may require reconstitution with deionized water. The water and a
glucose standard (100 mg/dL) are provided with the kit. Other materials required but
not provided are control samples to insure reproducibility and validity of the results.
Although this reagent may be used on automated and semi-automated systems, the
procedure outlined here is that of a manual procedure.
The process of specimen collection and preparation allows for either serum or
plasma determinations. If serum is to be used, it must be removed from the clot within
30 minutes of collection to prevent glycolysis (blood glucose will drop significantly
Copyright 2010 Cengage Learning. All Rights Reserved. May not be copied, scanned, or duplicated, in whole or in part. Due to electronic rights, some third party content may be suppressed from the eBook and/or eChapter(s).
Editorial review has deemed that any suppressed content does not materially affect the overall learning experience. Cengage Learning reserves the right to remove additional content at any time if subsequent rights restrictions require it.