CLINICAL LAB SCIENEC

424
ESSENTIALS OF CLINICAL LABORATORY SCIENCE
In the Lab
12. Discard all supplies used for the procedure in the appropriate containers.
A disinfectant should be used to clean the work surfaces and equipment should
be cleaned and restored to its former position. Gloves should be discarded
appropriately and the hands washed thoroughly in accordance with established
policies.
Reporting of Results
Interpret your results for ABO direct blood grouping according to the following
criteria.
Agglutination in Tube A = Group A
Agglutination in Tube B = Group B
Agglutination in Tubes A and B = Group AB
No agglutination in Tubes A or B = Group O
Tube A Tube B
Results + –
Tube A Tube B
Results – +
Tube A Tube B
Results + +
Tube A Tube B
Results – –
MANUAL IMMUNOHEMATOLOGY PROCEDURE #3
Direct Blood or Forward Grouping by Slide Method
Principles
The quality and care practiced by the laboratory professional is critical in the blood
bank, as a mistake has an immediate impact upon the patient in many instances.
These mistakes can be of misidentification, misinterpretation, or in the performance
of the testing. Slide tests, like tube tests for ABO grouping, employ the same technique
of introducing antigens on the surfaces of RBCs to anti-sera that contain
antibodies that correspond to the antigens for a specific blood group. If the antigen
that correlates with the antibody against that particular antigen is not present on the
RBCs, no reaction occurs.
The direct grouping performed on a microscope slide is the quickest and easiest
method to determine the ABO grouping (Figure 17-2) but is not the choice for most
blood banking professionals. The problem with this procedure lies in the possibility
of proteins interfering with the reaction, which is avoided in the tube method, as the
RBCs that contain the A or B antigen(s) are washed before testing.
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