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Análisis de la expresión de 103 ORFs de función desconocida en S. cerevisiae Blomberg, A. & Adler, L. (1989). Roles of glycerol and glycerol-3-phosphate dehydrogenase (NAD+) in acquired osmotolerance of Saccharomyces cerevisiae. J. Bacteriol., 171: 1087-1092. Boguslawski, G. (1992). PBS2, a yeast gene encoding a putative protein kinase, interacts with the RAS2 pathway and affects osmotic sensitivity of Saccharomyces cerevisiae. J. Gen. Microbiol., 138: 2425-2432. Bolivar, F. & Backman, K. (1979). Plasmids of Escherichia coli as cloning vectors. Methods Enrymol., 68: 245-267. Brewster, J.L., De Valoir, T., Dwier, N.D., Winter, E. & Gustin, M.C. (1993). An osmosensing signal transduction pathway in yeast. Science, 259: 1760-1763. Brown, A.J.P. (1995). Preparation of total RNA. In: Methods in Yeast Molecular Biology (Evans, I., ed.) pp 269-276.Humana Press, NY, USA. Burlini, N., Morandi, S., Pellegrini, R., Tortora, P. & Guerritore A. (1989). Studies on the degradative mechanism of phosphoenolpyruvate carboxykinase from the yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta, 1014: 153-161. Cadahía, J.L. (1996). Clonación del gen ROX6 de Saccharomyces cerevisiae. Tesis de licenciatura, Universidad de La Coruña. Capieaux, E., Vignais M.-L., Sentenac, A. & Goffeau, A. (1989). The yeast H±-Atpase gene is controlled by the promoter binding factor TUF. J. Biol. Chem., 264: 7437-7446. Carlson, M. & Botstein, D. (1982). Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase. Cell, 28: 145-154. Church, G.M. & Gilbert, W. (1984). Genomic sequencing. Proc. Natl. Acad. Sci. USA, 81: 1991-1995 . Coffman, J.A., Rai, R., Cunningham, T., Svetlov, V. & Cooper, T.G. (1996). Gat 1 p, a GATA family protein whose production is sensitive to nitrogen catabolite repression, participates in transcriptional activation of nitrogen-catabolic genes in Saccharomyces cerevisiae. Mol. Cell. Biol., 16: 847-858. Coffman, J.A., Rai, R., Loprete, D.M., Cunningham, T., Svetlov, V. & Cooper, T.G. (1997). Cross regulation of four GATA factors that control nitrogen catabolic gene expression in Saccharomyces cerevisiae. J. Bacteriol., 179: 3416-3429. Cohen, R., Holland, J.R., Yokoi, T. & Holland, M.J. (1986). Identification of a regulatory region that mediates glucose-dependant induction of the Saccharomyces cerevisiae enolase gene EN02. Mol. Cell. Biol., 6: 2287-2297. Crauwels, M., Winderickx, J., De Winde, J.H. & Thevelein, J. (1997). Identification of genes with nutrient-controlled expression by PCR-mapping in the yeat Saccharomyces cerevisiae. Yeast, 13: 973-984. 186 • •
• BIBLIOGRAFÍA Dang, V.-D., Bohn, C., Bolotin-Fukuhara, M. & Daighan-Fornier, B. (1996). The CCAAT box-binding factor stimulates ammonium assimilation in Saccharomyces cerevisiae, defining a new cross-pathway regulation between nitrogen and carbon metabolisms. J. Bacteriol., 178: 1842-1849. DeAntoni, A., D'Angelo, M., Dal Pero, F., Sartorello, F., Pandolfo, D., Pallaricini, A., Lanfranchi, G. & Valle, G. (1996). The DNA sequence of cosmid 14-13b from chromosome XIV of Saccharomyces cerevisiae reveals an unusually high number of overlapping open reading frames. Yeast, 13: 261-266. Decottignies, A. & Goffeau, A. (1997). Complete inventory of the yeast ABC proteins. Nature Genet., 15: 137-145. Delbruck, S. & Ernst, J.F. (1993). Morphogenesis-independent regulation of actin transcript levels in the pathogenic yeast Candida albicans. Mol. Microbiol., 10: 859-866. Denis, C.L., Ciriacy M. & Young, E.T. (1981). A positive regulatory gene is required for accumulation of the functional messenger RNA for the glucose-repressible alcohol dehydrogenase from Saccharomyces cerevisiae. J. Mol. Biol., 148: 355-368. Denis, C.L., Ferguson, J. & Young, E.T. (1983). mRNA levels for the fermentative^ alcohol dehydrogenase of Saccharomyces cerevisiae decrease upon growth on a nonfermentable carbon source. J. Biol. Chem., 258: 1165-1171. DeRisi, J.L. Iyer, V.R. & Brown, P.O. (1997). Exploring the metabolic^and genetic control of gene expression on a genomic scale. Science, 278: 680-686. Dubois, E. & Messenguy, F. (1997). Integration of the multiple controls regulating the expression of the arginase gene CAR1 of Saccharomyces cerevisiae in response to different nitrogen signals: role of Gln3p, ArgRp-Mcmlp, and Ume6p. Mol. Gen. Genet., 253: 568-580. Dujon B. (1996). The yeast genome proyect: what did we learn? Trends Genet., 12: 263- 270. Dujon, B., Alexandraki, D., Andre, B., Ansorge, W., Baladron, V., Ballesta, J.P., Banrevi, A., Bolle, P.A., Bolotin-Fukuhara, M., Bossier, P., et al. (1994). Complete DNA sequence of yeast ĉhromosome XI. Nature, 369: 3 71-3 78. . Einerhand, A.W., Voorn-Brouwer, T.M., Erdmann, R., Kunau, W.H. & Tabak, H.F. (1991). Regulation of transcription of the gene coding for peroxisomal 3-oxoacyl-CoA thiolase of Saccharomyces cerevisiae. Eur. J. Biochem., 200: 113-122. Ellwood, M.S. & Craig, E.A. (1984). Differential regulation of the 70K heat shock gene and related genes in Saccharomyces cerevisiae. Mol. Cell. Biol., 4: 1454-1459. Federoff, H.J., Eccleshall, T.R. & Marmur, J. (1983). Carbon catabolite repression of maltase synthesis in Saccharomyces carlsbergensis. J. Bacteriol., 156: 301-307. 187
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• • El presente trabajo: Análi
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* • Por las numerosas imperfeccio
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• • • ^ 1. INTRODUCCIÓN ÍND
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• • • ^ 4.4.1.2. ORFs para la
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• • • • A6^: absorbancia a
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Análisis de la expresión de 103 O
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3. MATERIALES Y MÉTODOS
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• • 3.1. Ceaas celulares: 3.1.1
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• MATERIALES Y MÉTODOS El YNB (Y
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• • • • MATERIALES Y MÉTOD
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• 3.3. Extracción del RNA: 3.3.1
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• • MATERIALES Y MÉTODOS d) Pa
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• • MATERIALES Y MÉTODOS mezcl
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• • MATERIALES Y MÉTODOS para
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No G^, Análisis de la expresión d
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Análisis de la e^resión de 103 OR
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