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Análisis de la expresión de 103 ORFs de función desconocida en S. cerevisiae Repetto, B. & Tzagoloff, A. (1990). Structure and regulation of KGD2, the structural gene for yeast dihydrolipoyl transsuccinylase. Mol. Cell. Biol., 10: 4221-4232. Richard, G.F., Fairhead, C. & Dujon, B. (1997). Complete transcriptional map of yeast chromosome XI in different life conditions. J. Mol. Biol., 268: 303-321. Rodríguez-Belmonte, E., Rodríguez-Torres, A., Tizón, B., Cadahía, J.L., González-Siso, M.I., Ramil, E., Becerra, M., González-Domínguez, M. & Cerdán, E. (1996). Sequence analysis of a lOKb DNA fragment from yeast chromosome VII reveals a novel member of the DnaJ family. Yeast, 12: 145-148. Ronne, H. (1995). Glucose repression in fungi. Trends Genet., 11: 12-17 Rosemblum-Vos, L. S. (1988). Isolation and characterization of mutations wich cause overexpression of the CYC7 gene in Saccharomyces cerevisiae. PhD thesis. State University of New York at Albany. USA. Roth, S., Neuman-Silberberg, F.S., Barcelo, G. & Schupbach, T. (1995). Cornichon and the EGF receptor signaling process are necessary for both anterior-posterior and dorsalventral pathern formation in Drosophila. Cell, 16: 967-978. Rothstein, R.J. & Sherman, F. (1980). Genes affecting the expression of cytochrome c in yeast: Genetic mapping and genetic interactions. Genetics, 94: 871-889. Ruis, H. & Schiiller, C. (1995). Stress signaling in yeast. Bioessays, 17: 959-965. . Salmon, J.-M., Piñon, R. & Gancedo, C. (1989). Isolation and characterization of mutants of Saccharomyces cerevisiae able to sporulate in the presence of glucose. J. Gen. Microbiol., 135: 203. ^ Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989). Molecular cloning: a láboratory manual (Ford, N., Nolan, C. & Fergusson, M. eds.). Cold Spring Harbor Laboratory Press, NY, USA. ^ Sanchez, Y. & Lindquist, S. (1990). HSP104 required for induced thermotolerance. Science, 248: 1112-1115. Schmidheini, T., M^sh, R. Graf, R. & Braus, G.H. (1990). A GCN4 protein recognition element is not sufficient for GCN4-dependent regulation of transcription in the AR07 promoter of Saccharomyces cerevisiae. Mol. Gen. Genet., 224: 57-64. Schul, W., Groenhout, B., Koberna, K., Takagaki, Y., Jenny, A., Manders, E.M., Raska, I., Van Driel, R., De Jong, L. (1996). The RNA 3' cleavage factors CstF 64 kDa and CPSF 100 kDa are concentrated in nuclear domains closely associated with coiled bodies and newly synthesized RNA. EMBO J., 15: 2883-2892. Schudiner, O.; Yanover, C. & Benvenisty, N. (1997). Computer analysis of the entire budding yeast genome for putative targets of the GCN4 transcription factor. Curr. Genet. 33: 16-20. 196 • • • •
BIBLIOGRAFÍA Schŭller, H.-J. & Entian, K.-D. (1987). Isolation and expression analysis of two yeast regulatory genes involved in the derepression of glucose-repressible enrymes. Mol. Gen. Genet., 209: 366-373. Schŭller, C., Brewster, J.L., Alexander, M.R., Gustin, M.C. & Ruis, H. (1994). The HOG pathway controls osmotic regulation of transcription via the stress response element (STRE) of the Saccharomyces cerevisiae CTTI gene. EMBO J., 13: 4382-4389. Sharp, P.M. & Li, W.-H. (1987). The codon Adaptation Index. A measure of directional synonymous codon usage bias, and its potential applications. Nucleic Acids Res. 15: 1281- 1295. Shena, M., Shalon, D., Davis, R.W. & Brown, P.O. (1995). Quantitative monitoring of gene expression patterns with a complimentary DNA microarray. Science, 270: 467-470. Silar, P., Butler, G. & Thiele, D.J. (1991). Heat shock transcription factor activates transcription of the yeast metallothionein gene. Mol. Cell. Biol., 11: 1232-1238. Smart, W.C., Coffman, J.A. & Cooper, T.G. (1996). Combinatorial regulation of the Saccharomyces cerevisiae CARI (arginase) promoter in response to multiple environmental signals. Mol. Cell. Biol., 16: 5876-5887. Sogin, S.J. & Saunders, C.A. (1980). Fluctuation in polyadenylate size and content in exponential- and stationary-phase cells of Saccharomyces cerevisiae. J. Bacteriol., 144: 74- 81. Sorger, P.K. (1991). Heat shock factor and the heat shock response. Cell, 65: 363-366. Sprague, G.F. & Cronan Jr., J.E. (1977). Isolation and characterization of Saccharomyces cerevisiae mutants defective in glycerol catabolism. J. Bacteriol., 129: 1335-1342. Stanbrough, M. & Magasanik, B. (1996). Two transcription factors, Gln3p and Nillp, use the same GATAAG sites to activate the expression of GAPl of Saccharomyces cerevisiae. J. Bacteriol., 178: 2465-2468. Stanbrough, M., Rowen, D.W. & Magasanik, B. (1996). Role of the GATA factors Gln3p and Nit2p of Saccharomyces cerevisiae in the expresion of the nitrogen-regulated genes. Proc. Natl. Acad. Sci. USA, 92: 9450-9454. Surosky, R.T.; Strich, R. & Exposito, R.E. (1994). The yeast UMES gene regulates the . stability of ineiotic mRNAs in response to glucose. Mol. Cell. Biol., 14: 3446-3458. Svetlov, V.V. & Cooper, T.G. (1995). Review: Compilation and Characteristics of dedicated transcription factors in Saccharomyces cerevisiae. Yeast, 11: 1439-1484. Termier, M. & Kalogeropoulos, A. (1996). Discrimination between fortuitous and biologically constrained open reading frames in DNA sequences of Saccharomyces cerevisiae. Yeast, 12: 369-384. Tettelin, H. et al. (1997). The nucleotide sequence of Saccharomyces cerevisiae chromosome VII. Nature, 387: 81-84. 197
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• • El presente trabajo: Análi
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* • Por las numerosas imperfeccio
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• • • ^ 1. INTRODUCCIÓN ÍND
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• • • ^ 4.4.1.2. ORFs para la
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• • • • A6^: absorbancia a
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Análisis de la expresión de 103 O
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3. MATERIALES Y MÉTODOS
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• • 3.1. Ceaas celulares: 3.1.1
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• MATERIALES Y MÉTODOS El YNB (Y
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• • • • MATERIALES Y MÉTOD
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• 3.3. Extracción del RNA: 3.3.1
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• • MATERIALES Y MÉTODOS d) Pa
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• • MATERIALES Y MÉTODOS mezcl
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• • MATERIALES Y MÉTODOS para
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No G^, Análisis de la expresión d
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Análisis de la e^resión de 103 OR
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,oa Densidad génica ^ (%1 u Análi
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