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Análisis de la exQresión de 103 ORFs de función desconocida en S cerevisiae Yin, Z., Smith, R.J. & Brown A.J.P. (1996). Multiple signalling pathways trigger the exquisite sensitivity of yeast gluconeogenic mRNAs to glucose. Mol. Microbiol., 20: 751- 764. Yoo, H.S. & Cooper, T.G. (1989). The DAL7 promoter consists of multiple elements that cooperatively mediate regulation of the gene's expression. Mol. Cell. Biol., 9: 3231-3243 Yoshikawa, A. & Isono, K. (1990). Chromosome III of Saccharomyces cerevisiae: an ordered clone bank, a detailed restriction map and analysis of transcripts suggest the presence of 160 genes. Yeast, 6: 383-401. Yoshikawa, A. & Isono, K. (1991). Construction of an ordered clone bank and systematic analysis of the whole transcripts of chromosome VI of Saccharomyces cerevisiae. Nucleic Acids Res., 9: 1189-1195. Zamaroczy, M. & Bernardi, G. (1986). The primary structure of the mitochondrial genome of Saccharomyces cerevisiae - a review. Gene, 47: 155-177. Zhang, L., Hach, A. & Wang, C. (1998). Molecular mechanism governing heme signaling in yeast: a higher-order complex mediates heme regulation of the transcriptional activator HAPI. Moll. Cell. Biol. 18: 3819-3828. ^^ Zitomer, R. S. & Lowry, C. V. (1992). Regulation of gene expression by oxygen in Saccharomyces cerevisiae. Microbiol. Rev., 56: 1-11. Zitomer, R.S., Limbach, M.P., Rodríguez-Torres, A.M., Balasubramanian, B., Deckert, J. & Snow, P.M. (1997). Approaches to the study of ROXI repression of the hypoxic genes in the yeast Saccharomyces cerevisiae. Methods: A companion to Methods Enzymol., 11: 279-288. 200 •
7. APÉNDICE
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• • El presente trabajo: Análi
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* • Por las numerosas imperfeccio
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• • • ^ 1. INTRODUCCIÓN ÍND
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• • • ^ 4.4.1.2. ORFs para la
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• • • • A6^: absorbancia a
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• •
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Análisis de la expresión de 103 O
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3. MATERIALES Y MÉTODOS
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• • 3.1. Ceaas celulares: 3.1.1
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• MATERIALES Y MÉTODOS El YNB (Y
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• • • • MATERIALES Y MÉTOD
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• 3.3. Extracción del RNA: 3.3.1
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• • MATERIALES Y MÉTODOS d) Pa
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• • MATERIALES Y MÉTODOS mezcl
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• • MATERIALES Y MÉTODOS para
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No G^, Análisis de la expresión d
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Análisis de la expresibn de 103 OR
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Análisis de la e^resión de 103 OR
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,oa Densidad génica ^ (%1 u Análi
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Page 176 and 177: O O ^--i O O N e O >^ M ^ ^r^-^ a o
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