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Análisis de la expresión de 103 ORFs de función desconocida en S. cerevisiae Johnston, M. (1996). Genome sequencing: the complete code for a eukariotic cell. Curr. Biol., 6: 500-503. Johnston, M. & Carlson, M. (1992). Regulation of carbon and phosphate utilization In: The molecular and cellular biology of the yeast Saccharomyces: Gene expression. (Jones, E. W., Pringle, J.R. & Broach, J.R. eds.) Vol. 2, pp 238-240. Cold spring harbor Laboratory press NY, USA. Johnston, M., Andrews, S., Brinkman, R., Cooper, J., Ding, H., Dover, J., Du, Z., Favello, A., Fulton, L., Gattung, S. et al. (1994). Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science, 265: 2077-2082. Kaback, D.B., Angerer, L. & Davidson, D. (1979). Improved methods for the formation and stabilisation of R-loops. Nucleic Acids Res. 6: 2499-2517. Kafatos, F.C., Jones, C.W. & Stratiadis, E.F. (1979). Determination of nucleic acid sequence, homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res., 7: 1541-1552. Kalogeropoulos, A. (1995). Automatic intron detection in nuclear DNA sequence of Saccharomyces cerevisiae. Yeast, 11: 555-565. Keleher, C.A., Goutte, C. & Johnson, A.D. (1988). The yeast cell-type-specific repressor alpha 2 acts cooperatively with a non-cell-type-specific protein. Cell, 53: 927-936. Keng, T. (1992). HAPI and ROXI form a regulatory pathway in the repression of HEM13 transcription in Saccharomyces cerevisiae. Mol. Cell. Biol., 12: 2616-2623. Kennedy, B.K., Gotta, M., Sinclair, D.A., Mills, K., McNabb, D.S., Murthy, M., Pak, S.M., Laroche, T., Gasser, S.M. & Guarente, L. (1997). Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell, 89: 381-391. Klein, P., Kanemisa, M. & Delisi, C. (1985). The detection and classification of membrane-spanning proteins. Biochem. Biophys. Acta, 815: 468-476. K1ein,C.J.L.; Olsson,L.; Ronnow,B.; Mikkelsen,J.D.; Nielsen,J. (1996). Alleviation óf glucose repression of maltose metabolism by migl disruption in Saccharomycescerevisiae. Appl. Environ. Microbiol., 62: 4441-4449. Kraakman, L.S., Gri^oen, G., Zerp, S., Groeneveld, P., Thevelein, J.M., Mager, W.H. & Planta, R.J. (1993). Growth-related expression of ribosomal protein genes in Saccharomyces cerevisiae. Mol. Gen. Genet., 239: 196-204. Kuchin, S., Yeghiayan, P. & Carlson, M. (1995). Cyclin-dependant protein kinase and cyclin homologs SSN3 and SSN8 contribute to transcriptional control in yeast. Proc. Natl. Acad. Sci. USA, 92: 4006-4010. 190 • • •
• BIBLIOGRAFÍA Lagunas,R. (1986). Misconceptions about the enrgy metabolism of Saccharomyces cerevisiae. Yeast, 12: 221-228. Larsson, K., Ansell, R., Erikson, P. & Adler, L. (1993). A gene encoding sn-glycerol 3phosphate dehydrogenase (NAD+) complements an osmosensitive mutant of Saccharomyces cerevisiae. Mol. Microbiol., 10: 1101-1111. Lauer, G.D., Roberts, T.M. & Klotz, L.C. (1977). Determination of the nuclear DNA content of Saccharomyces cerevisiae and implication for the organization of DNA in yeast chromosomes. J. Mol. Biol., 114: 507-526. Lee, F.-J.S., Lin, L.-W. & Smith J.A. (1990). A glucose-repressible'gene encodes acetyl- CoA hydrolase from Saccharomyces cerevisiae. J. Biol. Chem., 265: 7413-7418. Leidich, S.D. & Orlean, P. (1996). Gpil, a Saccharomyces cerevisiae protein that participates in the first step in glycosylphosphatidylinositol anchor synthesis. J. Biol. Chem., 271: 27829-27837. Li, L., Ernsting, B.R., Wishart, M.J., Lohse, D.L. & Dixon, J.E. (1997). A family of putative tumor suppressors is structurally and functionally conserved in humans and yeast. J. Biol. Chem., 272: 29403-29406. Liao, S.-M., Zhang, J., Jeffery, D.A., Koleseske, A.J., Thompson, C.M., Chao, D.M., Viljoen, M., Van Vuuren, H.J.K. & Young, R.A. (1995). A kinase-cyclin pair in the RNA polymerase II holoenzyme. Nature, 374: 193-196. Lillehaug, J.R. & Kleppe, K. (1975). Effects of salts and polyamines on T4 polynucleotide kinase. Biochemistry, 14: 1225-1230. Lindquist, S. (1981). Regulation of protein synthesis during heat shock. Nature, 293: 311- 314. Lindquist, S. & Craig, E.A. (1988). The heat-shock proteins. Annu. Rev. Genet., 22: 631- 677. Lloyd, A.T. & Sharp, P.M. (1992). Codons: A microcomputer program for codon usage analysis. J. Heredity, 83: 239-240. Lobo, Z., & Maitra, P.K. (1977). Genetics of yeast hexokinase. Genetics, 86: 727. Lombardia, L. (1996). Ariálisis de la expresión de 52 ORFs de función desconocida en Saccharomyces cerevisiae. Tesis de licenciatura. Universidad de la Coruña. Lombardo, A., Carine, K. & Scheffler, I.E. (1990). Cloning and characterization of the iron-sulfur subunit gene of succinate dehydrogenase from Saccharomyces cerevisiae. , J. Biol. Chem., 265: 10419-10423. Lowry, C.V. & Zitomer, R.S. (1984). Oxygen regulation of aerobic and anaerobic genes mediated by common factor in yeast. Proc. Natl. Acad. Sci. USA, 81: 6129-6133. 191
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• • El presente trabajo: Análi
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* • Por las numerosas imperfeccio
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• • • ^ 1. INTRODUCCIÓN ÍND
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• • • ^ 4.4.1.2. ORFs para la
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• • • • A6^: absorbancia a
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Análisis de la expresión de 103 O
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3. MATERIALES Y MÉTODOS
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• • 3.1. Ceaas celulares: 3.1.1
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• MATERIALES Y MÉTODOS El YNB (Y
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• • • • MATERIALES Y MÉTOD
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• 3.3. Extracción del RNA: 3.3.1
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• • MATERIALES Y MÉTODOS d) Pa
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• • MATERIALES Y MÉTODOS mezcl
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• • MATERIALES Y MÉTODOS para
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No G^, Análisis de la expresión d
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Análisis de la e^resión de 103 OR
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