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Análisis de la expresión de 103 ORFs de función desconocida en S. cerevisiae Feinberg, A.P. & Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem., 132: 6-13. Fink, G.R. (1987). Pseudogenes in yeast ? Cell, 49: 5-6. Flattery-O'Brien, J.A., Grant, C.M. & Dawes, I.W. (1997). Stationary-phase regulation of the Saccharomyces cerevisiae SOD2 gene is dependant on additive effects of HAP2/3/4/S and STRE binding elements. Mol. Microbiol., 23: 303-312. Fodor, S.P.A., Rava, R.P., Huang, X.C., Pease, A.C., Holmes, C.P. & Adams, C.L. (1993). Multiplexed biochemical assays with biological chips. Nature, 364: 555-556. Fondrat, C. & Kalogeropoulos, A. (1996). Approaching the function of new genes by detection of their potential upstream activation sequences in Saccharomyces cerevisiae: application to chromosome III. CABIOS, 12: 363-374. Forbes, A. & Lehmann, R. (1998}. Nanos and pumilio have critical roles in the development and function of Drosophila germline stem cells. Development, 125: 679-690. Foury, F. (1997). Human genetic deseases: a cross-talk between man and yeast. Gene, 195: 1-10. Freire-Picos, M.A., Hollenberg, C.P., Breuning, K.D. & Cerdán, M.E. (1995). Regulation of cytochrome c expression in the aerobic respiratory yeast Kluyveromyces lactis. FEBS letters, 360: 39-42. Fuge, E.K., Braun, E.L. & Werner-Washburne, M. (1994). Protein synthesis in long-term stationary-phase cultures of Saccharomyces cerevisiae. J. Bacteriol., 176: 5802-5813. Galibert, F., Alexandraki, D., Baur, A., et al. (1996). Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X. EMBO J., 15: 2031-2049. Gancedo, C. & Schwerzmann, N. (1976). Inactivation by glucose of phosphoenolpyruvate carboxikinase from Saccharomyces cerevisiae. Arch. Microbiol., 109: 221-225. Gancedo, J.M. (1992). Catabolite repression in yeast. Eur. J. Biochem., 206: 297-313. Gee, S., Krauss, S.W., Miller, E., Aoyagi, K., Arenas, J. & Conboy, J.G. (1997). Cloning of mDEAH9, a putative RNA helicase and mammalian homologue of Saccharomyces cerevisiae splicing factor Prp43. Proc. Natl. Acad. Sci. USA, 94: 11803-11807. Geissler, S., Pereira, G., Spang, A., Knop, M., Soues S., Kilmartin, J. & Schiebel, E. (1996). The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment. EMBO J. 15: 3899-3911. Goffeau, A. & Vassaroti, A. (1991). The European project for sequencing the yeast genome. Res. Microbiol., 142: 901-903. 188 • •
BIBLIOGRAFÍA Goffeau, A., Park, J., Paulsen, I.T., Jonniaux, J.L., Dinh, T., Mordant & P., Saier, M.H. Jr (1997). Multidrug-resistant transport proteins in yeast: complete inventory and phylogenetic characterization of yeast open reading frames with the major facilitator superfamily. Yeast, 13: 43-54. Greer, H., Penn, M., Hauge, B., & Galgoci, B. (1982). Control of aminoacid biosynthesis in yeast. Rec. Adv. Yeast Mol. Biol., 1: 122-142. Ha, N., Hellauer, K. & Turcotte, B. (1996). Mutations in target DNA elements of yeast HAPI modulates its transcriptional activity without affecting DNA binding. Nucleic Acids Res., 2.4: 1453-1459. Haarasilta, S. & Oura, E. (1975). On the activity and regulation of anaplerotic and gluconeogenetic enzymes during the growth process of baker's yeast. The biphasic growth. Eur. J. Biochem., 52: 1-7. Herruer, M.H., Mager, W.H., Doorenbosch, T.M., Wessels, P.L., Wassenaar, T.M. & Planta R.J. (1989). The extended promoter of the gene encoding ribosomal protein S33 in yeast consists of multiple protein binding elements. Nucleic Acids Res., 17: 7427-7439 Herskowitz, I., Rine, J. & Strathern, J. (1992). Mating-type determination mating-type interconversion in Saccharomyces cerevisiae. In: The molecular and cellular biology of the yeast Saccharomyces: Gene expression. (Jones, E.W., Pringle, J.R. & Broach, J.R. ed.) Vol. 2, pp 591-595. Cold spring harbor Laboratory press NY, USA. Hoheisel, J. (1997). Oligomer chip technology. Trends Biotech., 15: 465-469. Hope, I.A. & Struhl, K. (1985). GCN4 protein, synthesized in vitro, binds HIS3 regulatory sequences: implications for general control of amino acid biosynthetic genes in yeast. Cell, 43: 177-188. Huibregtse, J.M., Engelke, D.R. & Thiele, D.J. (1989). Copper-induced binding of cellular factors to yeast metallothionein upstream activation sequences. Proc. Natl. Acad. Sci. USA, 86: 65-69. Huie, M.A., Scott, E.W., Drazinic, C.M., Lopez, M.C., Hornstra, I.K., Yang, T.P. & Baker, H.V. (1992). Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiae. Mol. Cell. Biol., 12: 2690-2700. Igual, J.C., Matallana, E., Gonzalez-Bosch, C., Franco, L. & Perez-Ortin, J.E. (1991). A new glucose-repressible gene identified from the analysis of chromatin structure in deletion mutants of yeast SUC2 locus. Yeast, 7: 3 79-3 89. Johnson, A.D. (1992). A combinatorial regulatory circuit in budding yeast. In transcription regulation (de. S. McKnight and K. Yamamoto). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. pp. 975-1006. 189
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• • El presente trabajo: Análi
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* • Por las numerosas imperfeccio
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• • • ^ 1. INTRODUCCIÓN ÍND
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• • • ^ 4.4.1.2. ORFs para la
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• • • • A6^: absorbancia a
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Análisis de la expresión de 103 O
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3. MATERIALES Y MÉTODOS
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• • 3.1. Ceaas celulares: 3.1.1
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• MATERIALES Y MÉTODOS El YNB (Y
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• • • • MATERIALES Y MÉTOD
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• 3.3. Extracción del RNA: 3.3.1
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• • MATERIALES Y MÉTODOS d) Pa
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• • MATERIALES Y MÉTODOS mezcl
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• • MATERIALES Y MÉTODOS para
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