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Análisis de la expresión de 103 ORFs de función desconocida en S cerevisiae Nehlin, J.O., Carlberg, M. & Ronne, H. (1991). Control of yeast GAL genes by MIGI repressor: A transcriptional cascade in the glucose response. EMBO J., 10: 3373-3377. Nelissen, B., De Wachter, R. & Goffeau A. (1997). Classification of all putative permeases and other membrane plurispanners of the major facilitator superfamily encoded by the complete genome of Saccharomyces cerevisiae. FEMS Microbiology Reviews, 21: 113- 134. Niedenthal, R.K., Riles, L., Johnston, M. & Hegemann, J.H. (1996). Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast. Yeast, 12: 773-786. Niederberger, P., Miozzor, G. & Heutter, R. (1981). Biological role of the general control of aminoacid biosynthesis in Saccharomyces cerevisiae. Mol. Cell. Biol., 1: 584-593. Nieto-Sotelo, J., Wiederrecht, G., Okuda, A. & Parker, C. S. (1990). The yeast heat shock transcription factor contains a transcriptional activation domain whose activity is repressed under nonshock conditions. Cell, 62: 807-817. Nomura, M., Gourse, R. & Baughman, G. (1984). Regulation of the synthesis of ribosomes and ribosomal components. Ann. Rev. Biochem., 53: 75-117. Oliver, S. (1996). A network approach to the systematic analysis of yeast gene function. Trends Genet., 12: 241-242. Oliver, S.G., Van Der Aart, O.J., Agostoni-Carbone, M.L. Aigle, M. Alberghina, L., Alexandraki, D., Antoine, G., Anwar, R., Ballesta, J.P., Benit, P. et al. (1992). The complete DNA sequence of yeast chromosome III. Nature, 357: 38-46. Orlean, P., Leidish, S.D., Drapp, ^D.A., Colussi, P. & Braz, J. (1994). Isolation of temperature-sensitive yeast GPI-anchoring mutants. Med. Biol. Res., 27: 145-150. Ozcan, S., Johnston, M. (1996). Two different repressors collaborate to restrict expression of the yeast glucose transporter genes HXT2 and HX7'4 to low levels of glucose. Mol. Cell. Biol., 16: 5536-5545. Paoluzi, S., Minenkova, O. & Castagnoli, L. (1997). The genes encoding the transcription factor yta^ii)60, the g4p 1 protein and a putative glucose-transporter protein are contained in a 12.3 Kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII. Yeast, 13: 85-91 Park, H.O. & Craig, E.A. (1989). Positive and negative regulation of basal expression of a yeast HSP70 gene. Mol. Cell. Biol., 9: 2025-2033. Penn, M.D., Galcoci, B. & Greer, H. (1983). Identification of AAS genes and their regulatory role in general control of aminoacids biosynthesis in yeast. Procl. Natl. Acad. Sci. USA, 80: 2704-2708. Perlman, D. & Halvorson, H.O. (1981). Distinct repressible mRNAs for cytoplasmic and secreted yea ŝt invertase are encoded by a single gene. Cell, 25: 525-536. ^ 194 • ^
i BIBLIOGRAFÍA Persson, B. &. Argos, P. (1994). Prediction of transmembrane segments in proteins utilising multiple sequence alignments. J. Mol. Biol., 237: 182-192. Pfiefer, K. (1988). Coordinate regulation of cytochrome c genes in Saccharomyces cerevisiae by heme and HAPI. PhD Thesis, MIT, Cambridge, USA. Philippsen, P. et al. (1997). The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications. Nature, 387: 93-98. Piper, P.W. (1993). Molecular events associated with acquisition of heat tolerance by the yeast Saccharomyces cerevisiae. FEMS Microbiol. Rev., 11: 339-355. Piper, P., Cucran, B., Davies, M., Lockheart, A. &.Reid, G. (1986). Transcription of the phosphoglycerate kinase gene of Saccharomyces cerevisiae increases vvhen fermentation cultures are stressed by heat shock. Eur. J. Biochem., 161: 525-531. Planta, R.J., Brown, A.J., Cadahia, J.L., Cerdan, M.E., DeJonge, M., Gent, M.E., Hayes, A., Kolen, C.P.A.M., Lombardia, L.J., Sefton, M., Oliver, S.G., Thevelein, J., Tournu, H., Van Delft, Y.J., Verbart, D.J. & Winderickx, J. (1998). Transcript analysis of 250 novel yeast genes from chromosome XIV. Yeast. Enviado. Polakis, E.S., Bartley, W. & Meek, G.A.(1964). Changes in the structure and enzyme activity of Saccharomyces cerevisiae in response to changes in the environment. Biochem. J., 90: 369-374. Polakis, E. S. & Bartley, W. (1966). Changes in dry weight, protein, deoxyribonucleic acid, ribonucleic acid and reserve and structural carbohydrate during the aerobic growth cycle of yeast. Biochem. J., 98: 883-887. Praekelt, U.M. & Meacock, P.A. (1990). HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function. Mol. Gen. Genet., 223: 97-106. Pringle, J.R. & Hartwell, L.H. (1981) .In: The molecular biology of the yeast Saccharomyces: life, cycle and inheritance (Strathern, S.N., Jones, E.W. & Broach, J.R. eds.) pp 97-142. Cold Spring Harbor Laboratory Press, NY, USA. ^ Quandt, K., Frech, K., Karas, H., Wingender, E. & Werner, T. (1995). MatInd and MatInspector- New fast and versatile tools for detection of consensus matches in nucleotides sequence data. Nucleic Acids Res., 23: 4878-4884. Rai, R., Genbauffe, F.S., Sumrada, R.A. & Cooper, T.G. (1989). Identification of sequences responsible for transcriptional activation of the allantoate permease gene in Saccharomyces cerevisiae. Mol. Cell. Biol., 9: 602-608. 195
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• • El presente trabajo: Análi
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* • Por las numerosas imperfeccio
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• • • ^ 1. INTRODUCCIÓN ÍND
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• • • ^ 4.4.1.2. ORFs para la
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• • • • A6^: absorbancia a
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• •
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Análisis de la expresión de 103 O
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• • •
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3. MATERIALES Y MÉTODOS
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• • 3.1. Ceaas celulares: 3.1.1
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• MATERIALES Y MÉTODOS El YNB (Y
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• • • • MATERIALES Y MÉTOD
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• 3.3. Extracción del RNA: 3.3.1
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• • MATERIALES Y MÉTODOS d) Pa
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• • MATERIALES Y MÉTODOS mezcl
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• • MATERIALES Y MÉTODOS para
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No G^, Análisis de la expresión d
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Análisis de la e^resión de 103 OR
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,oa Densidad génica ^ (%1 u Análi
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