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Análisis de la expresión de 103 ORFs de función desconocida en S cerevisiae Lundin, M., Nehlin, J.D. & Ronne, H. (1994). Importance of a flanking AT-rich region in target site recogñition by the GC-box-binding zinc finger protein MIGI. Mol. Cell. Biol., 14: 1979-1985. Magasanik, B. (1992). Regulation of nitrogen utilization in: The molecular and cellular biology of the yeast Saccharomyces: Gene expression. (Jones, E.W., Pringle, J.R. & Broach, J.R. eds.) Vol. 2, pp 283-317. Cold spring harbor Laboratory press, NY, USA. Mager, W.H. & Moradas Ferreira, P. (1993). Stress response of yeast. Biochem. J., 290: 1- 13. Mager, ^ W.H. & De Kruijff, A.J. (1995). Stress-induced transcriptional activation. Microbiol. Rev., 59: 506-531. Mager, W.H., Maurer, K. & Piper, P. W. (1998). Identifying stress genes. In "Methods in Microbiology: Yeast Gene Analysis" (Brown, A.J.P. & Tuite, M.F., eds.), pp 337-349. Academic Press, London, UK. Mahler, M.R., Jaynes, P.K., McDonough, J.P. & Hanson, D.K. (1981). Current topics in cellular regulation. (Horeker, B.L. & Stadtman, E.R. eds.), vol. 18, pp 454-474. Marchler, G., Schŭller, C., Adam, G. & Ruis, H. (1993). A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J., 12: 1997-2003. Martínez-Pastor, M.T., Marchler, G., Schuller, C., Marchler-Bauer, A., Ruis, H. & Estruch F(1996). The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). EMBO. J.; 15: 2227-2235. ^ Marzluf, G.A. (1997). Genetic regulation of nitrogen metabolism in the fungi. Microbiol. Mol. Biol. Rev., 61: 17-32. McDaniel, D., Caplan, A.J., Lee, M.-S.; Adams, C.C., Fishel, B.R., Gross, D.S. & Garrard, W.T. (1989). Basal-level expression of the yeast HSP82 gene requires a heat schock regulatory element. Mol. Cell. Biol., 9: 4789-4798. Mercado, J.J., Smith, R., Sagliocco, F.A., Brown, A.J.P. & Gancedo J.M. (1994). The levels of yeast gluconeogenic mRNAs respond to environmental factors. Eur. J. Biochem., 224: 473-481. Messing, J. (1979). A multipurpose cloning system based on stringle-stranded DNA bacteriophage M13. Recomb. DNA Tech. Bull., 2: 43. Mewes, H.W., Albermann, K., Heumann, K., Liebls, S. & Pfeiffer, F. (1997a). MIPS: A database for protein sequence, homology data and yeast genome information. Nucleic Acids Res., 25: 28-30. 192 • •
BIBLIOGRAFÍA Mewes, H.W., Albermann, K., B^hr, M., Frishman, D., Gleissner, A., Hani, J., Heumann, K., Maier, L.Á., Oliver, S.G., Pfeiffer, F. & Zollner, A. (1997b). Overview of the yeast genome. Nature, 387: 7-65. Miller, M.J., Xuong, N.-H. & Geiduschek, E.P. (1982). Quantitative analysis of the heat schock response of Saccharomyces cerevisiae. J. Bacteriol., 151: 311-327. Miller, S.M. & Magasanik, B. (1991). Role of the complex upstream region of the GDH2 gene in nitrogen regulation of the NAD-linked glutamate dehydrogenase in Saccharomyces cerevisiae. Mol. Cell. Biol., 11: 6229-6247. Minehart, P.L. & Magasanik, B. (1992). Sequence of the GLNl gene of Saccharomyces cerevisiae: Role of the upstream region in regulation of glutamine synthetase expression. J. Bacteriol., 174: 1828-1836. Mitchell, P., Petfalski, E., Shevchenko, A., Mann, M. & Tollervey, D. (1997). The exosome: A conserved eukaryotic RNA processing complex containing mutiple 3'-^5' Exoribonucleases. Cell, 91: 457-466. Moehle, C.M. & Jones, E.W. (1990). Consequences of growth media, gene copy number and regulatory mutations on the expression of the PRBI gene of Saccharomyces cerevisiae. Genetics, 124: 39-55. Moehle, C.M. & Hinnebush, A.G. (1991). Association of RAP1 binding sites with stringent control of ribosomal protein gene transcription in Saccharomyces cerevisiae. Mol. Cell. Biol., 11: 2723-2735. Moesch, H.U., Graf, R., Schmidheini, T. & Braus, G. (1990). Three GCN4 responsive elements act synergistically as upstream and as TATA-like elements in the yeast TRP4 promoter. EMBO J., 9: 2951-2957. Mortimer, R.K. & Schild, D. (1989). Genetic map of Saccharomyces cerevisiae. Edition 10. Yeast, 5: 321-403. Mullis, K.B. & Faloona, F.A. (1987). Specific synthesis of DNA in vitro via a polymerasecatalysed chain reaction. In: Methods in Enzymology (Wu, R. ed.), Vol. 155, pp 335-350. Academic press, San Diego, CA, USA. Naitou, M., Hagiwara, H., Hanaoka, F., Eki, T. & Murakami, Y. (1997). Expression profiles of transcripts from 126 open reading frames in the entire chromosome VI of Saccharomyces cerevisiae by systematic northern analyses. Yeast, 13: 1275-1290. Nakai, K. & Kanehisa, M. (1992). A knowledge base for predicting protein localization sites in eukaryotic cells. Genomics, 14: 897-911. Nasr, F., Becam, A.M. & Herbert, C.J. (1996). The sequence of 36.8 Kb from the left arm of chromosome XIV reveals 24 complete open reading frames: 18 correspond to new genes, ^ one of wich encodes a protein similar to the human myotonic distrophy kinase. Yeast, 12 :169-175. 193
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• • El presente trabajo: Análi
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* • Por las numerosas imperfeccio
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• • • ^ 1. INTRODUCCIÓN ÍND
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• • • ^ 4.4.1.2. ORFs para la
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Análisis de la expresión de 103 O
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3. MATERIALES Y MÉTODOS
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• • 3.1. Ceaas celulares: 3.1.1
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• MATERIALES Y MÉTODOS El YNB (Y
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• • • • MATERIALES Y MÉTOD
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• 3.3. Extracción del RNA: 3.3.1
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• • MATERIALES Y MÉTODOS d) Pa
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• • MATERIALES Y MÉTODOS mezcl
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• • MATERIALES Y MÉTODOS para
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