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Análisis de la expresión de 103 ORFs de función desconocida en S. cerevisiae Tizón, B. (1997). Secuenciación de un fragmento de DNA del cromosoma VII de Saccharomyces cerevisiae y análisis funcional de las ORFs YGL131c, YGL129c, YGL125w, YGL124c, e YGL121c. Tesis de doctorado. Universidad de La Coruña. Tizón, B., Rodríguez-Torres, A, Rodríguez-Belmonte, E., Cadahía, J.L. & Cerdán, E. (1996). Identification of a putative methylenetetrahydrofolate reductase by sequence analysis of a 6.8Kb DNA fragment of yeast chromosome VII. Yeast, 12: 1047-1051. Treitel, M.A. & Carlson, M. (1995). Repression by SSN6-TUPI is directed by MIGI, a represor/activator protein. Proc. Natl. Acad. Sci. USA, 92: 3132-3136. Trumbly, R.J. (1986). Isolation of Saccharomyces cerevisiae mutants constitutive for invertase synthesis. J. Bacteriol., 166: 1123-1127. Trumbly, R.J. (1992). Glucose repression in the yeast Saccharomyces cerevisiae. Mol. Microbiol., 6: 15-21. Tugendreich, S., Basset Jr., D.E., Mc Kusick, V.A., Boguski, M. S. & Hieter, P. (1994). Genes conserved in yest and humans. Human molecular genetics, 3: 1509-1517. Tzamarias, D. & Struhl, K. (1995). Functional disection of the yeast Cyc8-Tupl transcriptional corepressor complex. Nature, 369: 758-761. Valdés-Hevia, M.D., De La Guerra, R. & Gancedo, C. (1989). Isolation and characterization of the gene encoding phosphenolpyruvate carboxykinase from Saccharomyces cerevisiae. FEBS Lett., 258: 313-316. Varela, J.C.S., Praekelt, U.M., Meacock, P.A., Planta, R.J. & Mager, W.H. (1995) The Saccharomyces cerevisiae HSP 12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A. Molecular and Cellular Biology, 15: 6232-6245. Velculescu, V.E., Zhang, L., Vogelstein, B. & Kinzler, W.K. (1995). Serial analysis of gene expression. Science, 270: 484-487. Velculescu, V.E., Zhang, L., Zhou, W., Vogelstein, J., Basrai, M.A., Bassett Jr., D.E., Hieter, P., Vogelstein, B. & Kinzler, W.K. (1997). Characterization of the yeast transcriptome. Cell, 88: 243-251. Vitale, G., Fabre, E. & Hurt, E.C. (1996). NBP35 encodes an essential and evolutionary conserved protein in Saccharomyces cerevisiae with homology to a superfamily of bacterial ATPases. Gene, 178: 97-106. Wahi, M. & Johnson, A.D. (1995). Identification of genes required for alfa2 repression in Saccharomyces cerevisiae. Genetics, 140: 79-90. Walsh, M.C.; Scholte, M.; Valkier, J.; Smits, H.P. & Vandam,K. (1996). Glucose sensing and signaling properties in Saccharomyces cerevisiae require the presence of at least 2 members of the glucose-transporter family. J. Bacteriol., 178: 2593-2597. 198 •
BIBLIOGRAFÍA Weaver, P.L., Sun, C. & Chang, T.H. (1997). Dbp3p, a putative RNA helicase in Saccharomyces cerevisiae, is required for efficient pre-ribosomal-RNA processing predominantly at site a(3). Mol. Cell. Biol., 17: 1354-1365. Werner-Washburne, M., Braun, E., Johnston, G.C. & Singer, R.A. (1993). Stationary phase in the yeast Saccharomyces cerevisiae. Microbiol. Rev., 57: 383-401. Werner-Washburne, M., Braun, E.L., Crawford, M.E. & Peck, V.M. (1996). Stationary phase in Saccharomyces cerevisiae. Mol. Microbiol., 19: 1159-1166. Wiederrecht, G., Shuey, D.J., Kibbe, W.A & Parker, C.S. (1987). The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties. Cell, 48: 507-515. Willett, C.E., Gelfman, C.M. & Holland, M.J. (1993). A complex regulatory element from the yeast gene EN02 modulates GCRI-dependent transcriptional activation. Mol. Cell. Biol., 13: 2623-2633. Wilson, K.L. & Herskowitz, I. (1984). Negative regulation of STE6 gene expression by the a2 product of Saccharomyces cerevisiae. Mol. Cell. Biol., 4: 2420-2427. Wingender, E., Kel, A.E., Kel, O.V., Karas, M., Heimeyert, T., Dietze, P., Knueppel, R., Romaschenko, A.G. & Kolchanov, N.A. (1997). TRANSFAC, TRRD, and COMPEL: Towards a federated database system on transcriptional regulation. Nucl. Acid. Res., 25: 265-268. Winston, F., Dollard, C. & Ricupero-Hovasse, S.L. (1995). Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast, 11: 53-55. Wodicka, K., Dong, H., Mittmann, M., Ho, M. & Lockheart, D.J. (1997). Genome-wide expression monitoring in Saccharomyces cerevisiae. Nature Biotech., 15: 1359-1367. Wolfe, K. & Shields, D. (1997). Molecular evidence for an ancient duplication of the entire Saccharomyces cerevisiae genome. Nature, 387: 708-713. Woods, R.A., Roberts, D.G., Stein, D.S. & Filpula, D. (1984). Adenine phosphoribosyltransferase mutants in Saccharomyces cerevisiae. Gen. Microbiol., 184: 2629-2657. Wright, R.M. &. Poyton, R.O. (1990). Release of two Saccharomyces cerevisiae cytochrome genes, COX6 and CYCI, from glucose repression requires the SNFI and SSN6 gene products. Mol. Cell. Biol., 10: 1297. Xing, Y., Fikes, J.D. & Guarente, L. (1993). Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain. EMBO J., 12: 4647-4655. Yang, S.S., Wei, Y.L., Sung, T.F. (1991).Chemical analysis and amino acid content of temperature-sensitive mutants of Saccharomyces cerevisiae. Chung Hua Min Kuo Wei Sheng Wu Chi Mien I Hsueh Tsa Chih 24: 311-320. 199
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• • El presente trabajo: Análi
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* • Por las numerosas imperfeccio
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• • • ^ 1. INTRODUCCIÓN ÍND
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• • • ^ 4.4.1.2. ORFs para la
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Análisis de la expresión de 103 O
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3. MATERIALES Y MÉTODOS
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• • 3.1. Ceaas celulares: 3.1.1
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• MATERIALES Y MÉTODOS El YNB (Y
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• • • • MATERIALES Y MÉTOD
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• 3.3. Extracción del RNA: 3.3.1
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• • MATERIALES Y MÉTODOS d) Pa
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• • MATERIALES Y MÉTODOS mezcl
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• • MATERIALES Y MÉTODOS para
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Análisis de la e^resión de 103 OR
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