UNIVERSIDAD COMPLUTENSE DE MADRID FACULTAD DE ...

8. SUMMARY
material for clamping the mesenteric artery, causing of ischaemia.
The infusion of SOD or SS, was carried out by IV injection through an infusion
microsystem composed by venous catheter Abocath (num. 24), to be inserted in one of
te marginal veins of the rabbit ear and a insulin syringe coupled to a precision
microinjector able to supply the established dose for each animal in the exact time for
each experience (10 minutes).
fle lipid peroxidation of the intestinal segments was studied carrying out the
TBA test, with estándar laboratory equipment and a spectrophotometer with reading
capacity at the band of 535 nm. The system was calibrated with known samples of
MDA, and a regression pattem line was drawing to be compared with the problem
samples.
The necropsy of dead or euthanasied animals were carried out systematically,
obtaiing samples from the intemal organs, to be later histopatologicaly examined in the
Service of Pathological Anatomy of the Hospital Gómez Ulla.
The statistical study, was made for the survival series by the method of Kaplan-
Meier, using the test of square Chi to obtain the comparative values of the distribution,
the P ofPearson and the significance degree for an unilateral error ofp = 0,05. Student
T test was used (for matched samples and an unilateral error of p = 0,05) for the clinical
assay testing the lipid peroxidation by determination ofthe quantitative levels of MDA,
Results and discussion
The survival time in animals subjected to ischemia of the AMC, decreases in a
proportional and significant relation (y,