Program including abstracts as pdf available here
Program including abstracts as pdf available here
Program including abstracts as pdf available here
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
OP 24<br />
Title<br />
A CEPHALOMETRIC MICROCOMPuTER TOMOGRAPH (MCT) STuDy ON MICE<br />
Authors<br />
F. JuHASz 1 , b. SzAbO 1 , CS. DObO NAGy 1 , D. GyORI 2 , A. MOCSAI 2 , G. FAbIAN 3<br />
Affiliations<br />
1 Semmelweis University, Oral Diagnostic Institute, Budapest, HUNGARY, 2 Semmelweis University,<br />
Department of Physiology, Budapest, HUNGARY, 3 Semmelweis University, Department of<br />
Pedodontics and Orthodontics, Budapest, HUNGARY<br />
Body<br />
Objectives: The aim of this study w<strong>as</strong> to examine synostosis, cranial b<strong>as</strong>e- length and skull<br />
<strong>as</strong>ymmetry in PLCγ2 knock-out mice <strong>as</strong> a model for osteopetrosis.<br />
Methods: In this study, four 135-days-old and one 24-days-old Phospholypace C gamma 2<br />
(PLCγ2) knockout mice – and wild-type mice for comparison– were examined. The newborn<br />
mice were tested by PCR, then Western blot h<strong>as</strong> showed PLCγ2 expression. The length of the<br />
cranial b<strong>as</strong>e and the <strong>as</strong>ymmetry of the skull were me<strong>as</strong>ured by MCT (60 kV, 0,5° rotation step,<br />
14 µm voxel size). Hystomorphometric analysis w<strong>as</strong> used to demonstrate the developmental<br />
stage of the synostosis while double staining with Alcian Blue and Alizarin Red S indicated the<br />
synchondrosis and the ossification centers, respectively. Morphologic parameters were determined<br />
from 3D rendered images using DataViewer (Skyscan) software.<br />
Results: T<strong>here</strong> w<strong>as</strong> a significant length-difference (p
Change language