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OP 66
Title
THE IMPACT OF CbCT ON EXPRESSION OF SELECTED GENES IN THE ORAL MuCOSA
Authors
T. KuLCzyK 1 , M. DySzKIEWICz-KONWINSKA 1
aBSTracTS OP 6
Affiliations
1 Section of Dental Radiology,Department of Biomaterials,Poznan University of Medical
Sciences, Poznan, POLAND
Body
Introduction: The effects of low dose radiation on human cells are disputed. Some non-stochastic
effects may be related to DNA mutations but some studies which suggest that significant
differences in gene expression following radiation exposure may also play an important
role. Health risk estimations are based on high dose radiation studies. These findings cannot
be directly transferred to low dose radiation risk evaluation. Therefore selecting genes that
are specific to low level radiation, and analyzing them in patients undergoing diagnostic procedures,
might be helpful in creating a model of health risk associated with low level radiation.
Material and method: Analysis of selected gene expression, (Grap 2,Fdxr,Cdkn1a) in epithelial
cells of the oral mucosa was performed in five patients who underwent CBCT examination
of the maxilla-facial region (Scanora3Dx). Brush biopsy samples of cheek mucosa were taken,
immediately after CBST examination and then 6,12,24,72 hours and 7 days after the examination.
The total RNA was isolated according Chomczynski and Sacchi`s method. The RNA
samples were then treated with DNase I, and reversely transcribed into cDNA using hexamer
and oligo-dT primers. Quantitative analyses of the three genes were performed by PCR Array.
Results: Changes in expression of the analyzed genes were observed. There was an increase in
the expression in each successive sample from each patient until 48 hours after the examination
when expression started to drop. but did not return to original levels.
Conclusions: The analyzed genes were subject to substantial changes due to radiation caused
by CBCT examination.
Keywords
CBCT, gene expression
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