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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Introgression lines of wild Pisum fulvum in cultivated pea (P. sativum) genetic
background, as a tool to broaden genetic diversity.
Petr Smýkal 1 , Oleg Kosterin 2
1 Department of Botany, Palacký University in Olomouc, Olomouc, Czech Republic
2 Institute of Cytology and Genetics, Siberian Department of Russian Academy of Sciences, Novosibirsk, Russia
Plant evolution under domestication has altered numerous traits, including self-pollination which
reinforced fertility barriers between wild and cultivated populations, facilitating fixation of the
desired genotype. Consequently, domestication bottleneck has resulted in high degree of
relatedness between varieties, which was further pronounced in modern breeding programs,
leading to narrower genetic base of cultivated germplasm, prone to pests and diseases. The study
of genetic diversity preserved in pea collections showed that although wide diversity is captured
among cultivated material, wild material provides yet broader diversity. We report development
of 105 chromosome segment substitution BC 2S 3 lines (CSSL) containing genomic segments of
phylogenetically the most distant wild pea (Pisum fulvum WL2140) in the cultivated pea (P. sativum
subsp. sativum WL1238) genetic background. Selected polymorphic 28 microsatellite and 44 genespecific
markers covered all seven linkage groups (LG) of pea 4.45 Gbp genome at 2 to 82 cM
spacing, with mean of 15.4 cM, delimiting 78 chromosomal regions. The heterozygozity was
detected in 533 (8%) cases while recurrent Pisum sativum parent in 4552 (69%) and introgressed
segments of P. fulvum in 1551 (23%) of 40 cM in average, covering 20 – 70% of given linkage
group. There were 5 to 14 segments per line, with mean of 9.6. These lines have been
phenotyped for 14 traits (including branching, height, node, pod and seed numbers) showing
often transgression. A CSSL Finder analysis was used to reveal genetic and graphical relationships
and select lines. Establishment of such permanent introgression library will allow phenotypic
characterization of unlimited number of target traits, which, coupled together with higher density
markers, will provide means for QTL and gene identification and subsequent incorporation in
desired genotypes ultimately leading to better performing pea varieties.
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