here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Functional exploration of salt responsive Phaseolus vulgaris (common bean) genes using
model organism Arabidopsis thaliana
Balkan Canher 1 , Mahmutcan Hız 1 , Harun Niron 1 , Roberta Nurcato 2 , Giorgia Batelli 2 , Stefania
Grillo 2 , Muge Turet Sayar 1
1 Bogazici University, Istanbul, Turkey
2 CNR-IGV, Naples, Italy
Phaseolus vulgaris is a widely cultivated leguminous crop that is highly susceptible to soil salinity.
Pre-requisite for the development of salt-tolerant crops is the identification of key genetic
determinants between tolerant and susceptible germplasm in response to salt stress. To this end a
subtraction suppressive hybridization (SSH) cDNA library was constructed using mRNA from
susceptible TR43477 and tolerant ISPIR genotype. The construction of the root library yielded
around 30000 colonies and leaf library around 800 colonies including transcripts such as
aquaporins, bZIP transcription factors, vacuolar ATPases. 7 genes from SSH library and 9 extra
genes which are not from the SSH library but are known to be involved in salinity response were
selected as candidate genes for functional study. For qPCR analysis GAPDH and Cyclophilin
were validated as stably expressed reference genes for salt treatment experiments. qPCR analysis
showed 10 out of 16 genes were significantly upregulated. (p