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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Association of SNP markers with pea grain protein and grain yield traits in Italy:
preliminary results
Barbara Ferrari 1 , Paolo Annicchiarico 1 , Gregoire Aubert 2 , Karen Boucherot 2 , Judith Burstin 2 ,
Myriam Huart-Naudet 2 , Anthony Klein 2 , Massimo Romani 1
1Consiglio
per la Ricerca e la Sperimentazione in Agricoltura, Fodder and Dairy Productions Research Centre,
Lodi, Italy
2
Institut National de la Recherche Agronomique, Unité Mixte de Recherches en Génétique et Ecophysiologie des
Légumineuses à Graines, Dijon, France
Grain yield and quality are the main economically important traits in breeding of field pea (Pisum
sativum L.). The objective of our work was investigating trait-marker associations in elite pea
germplasm, with the final aim to develop marker-assisted selection procedures for these traits.
The genetic base for this study was represented by three connected recombinant inbred line
(RIL) populations obtained from paired crosses between three genotypes (the varieties Attika,
Isard and Kaspa) which featured different geographical origin and outstanding adaptation to
Italian cropping environments. At least 86 F 7 lines per RIL generated by single-seed descent were
evaluated for grain yield, plant height, lodging resistance and grain protein content in a springsown
field experiment in Lodi. The same material was genotyped by 384 SNP markers. Values
for protein content, estimated by NIRS, showed range values at least as large as 6% in each RIL
population. SNP genotyping helped in building a genetic consensus map and detecting QTLs
related to all phenotypic traits. Further work is on-going for enlarging both the evaluation
environments for phenotyping and the set of SNP markers for map saturation.
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