here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Marker development for anthracnose resistance in narrow-leafed lupin via transcriptome
profiling
B Ruge-Wehlin 1 , K Fischer 1 , R Dieterich 2 , B Rotter 3 , P Winter 3 , P Wehling 1
1 Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Groß Lüsewitz, Germany
2 Saatzucht Steinach GmbH & Co. KG, Bocksee, Germany
3 GenXPro GmbH, Frankfurt / Main, Germany
Anthracnose, caused by the fungus Colletotrichum lupini, represents the most important disease in
lupin cultivation worldwide. To obtain high and stable yields it is necessary to plant resistant
cultivars as there are no possibilities to overcome the fungus via pesticides. In a screening of plant
genetic resources of Lupinus angustifolius we obtained a completely resistant breeding line, carrying
a dominant resistance gene as revealed by genetic analyses of F 2 populations. Those F 2
populations are the basis for the development of molecular markers. Three approaches shall be
applied to genetically map the resistance gene and to generate molecular markers suited as
selection tools; (I) use of anchor markers already mapped in L. angustifolius, (II) use of sequence
information drawn from model genomes and (III) use of sequence information of differentially
expressed L. angustifolius cDNA. cDNA sequencing was done with the resistant and susceptible
parents of the F 2 mapping population, both inoculated with the fungus, resulting in 7 477 070
sequence tags with a mean sequence length of 84 bp assembled to 577 contigs with a mean contig
size of 486 bp. Finally informative SNPs were identified. Part of the SNP sequences is annotated
to SwissProt which enables sequence filtering by their function concerning putative resistancemediating
proteins. Based on this information, 116 primer pairs have been developed so far. 73
% of these proved to be polymorphic between the parents, which could be demonstrated by
high-resolution melt analysis of amplicons. To-date, 22 SNPs has been integrated in the genetic
map, which consists of 20 linkage groups and 4 single clusters with 369 loci and a genetic length
of 1887.2 cM. Linkage analysis suggests the resistance to be located on linkage group NLL-11.
Additional efforts are necessary to find markers more closely linked to the resistance locus Lanr Bo .
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