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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Saturation of a Quantitative Trait Loci controlling resistance to Orobanche crenata in pea
María José Cobos 1 , Diego Rubiales 1 , Sara Fondevilla 1,2
1 Institute for Sustainable Agriculture – CSIC, Córdoba, Spain
2 University of Frankfurt, Frankfurt am Main, Germany
Crenate broomrape (Orobanche crenata) is a root parasitic weed that represents a major constraint
for pea cultivation in the Mediterranean Basin and Middle East. In a previous study we identified
several Quantitative Trait Loci (QTLs) associated with resistance to O. crenata in the pea RIL
population P665 x Messire. A QTL located in LGVII was associated with a lower number of O.
crenata tubercles per root length and explained a high proportion of the phenotypic variation of
the trait (33%). In this study this genomic region was saturated with new markers by comparative
mapping with Medicago truncatula. Nine molecular markers previously mapped in P. sativum LGVII
and in M. truncatula were analysed in P665 x Messire. Eight of them could be included in the map,
being dFPA2maker located into de QTL. In addition, a SCAR marker (SCARK6) was developed
from a RAPD marker tightly linked to the QTL. The inclusion in our map of these markers and
the M. truncatula EST DNABP allowed the comparison with M. truncatula. The pea region
containing the QTL was located in M. truncatula chromosome 4. Thirty eight M. truncatula genes,
expected to be located into the QTL region, were selected. Four of them could be mapped in
LGVII in P665 x Messire genetic map. Two genes (Medtr4g068580.1 and Medtr4g068270.1) colocalized
with the QTL.
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