here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Inheritance of rust resistance in lentil (Lens culinaris)
Sarvjeet Singh 1 , Ashok Kumar 1 , RK Gill 1 , F Mekonnen 2 , Alok Kumar 1 , TR Sharma 3 , Shiv Kumar 2 ,
Seid Ahmed 2 , A Sarker 2
1 Department of Plant Breeding & Genetics, Punjab Agricultural University, Ludhiana, India
2 International Center for Agricultural Research in the Dry Areas, Aleppo, Syria
3 Department of Agricultural Biotechnology, CSK Himachal Pradesh Agricultural University, Palampur, India
Lentil (Lens culinaris L. Medikus) is an important cool season grain legume of South Asia with 34
and 46% share in global production and area. In India alone, it is grown on 1.46 m ha area with
0.99 Mt production. Lentil rust caused by Uromyces viciae-fabae is a widespread disease in North India
causing 30-60% yield losses. There are contradictory reports on number (mono or digenic) and
nature (dominant or recessive) of rust resistance gene(s). The aim of the present study is to work
out the genetics of rust resistance. A widely adapted rust susceptible Indian cultivar, L9-12, was
crossed as female with a rust resistant advance breeding line, FLIP2004-7L, from ICARDA, Syria.
The F 1, F 2 and F 6 RILs were grown at Gurdaspur (a hot-spot for rust) to screen for rust reaction
under natural field conditions. Data on reaction to rust were recorded on 1-9 rating scale, where
1=highly resistant and 9=highly susceptible. The plants/progenies showing 3 were categorized as susceptible. All the F 1 plants
gave susceptible reaction to rust, while out of 169 F 2 plants, 120 were susceptible and 49 were
resistant and thus gave good fit to the 3:1 ratio (χ 2 =1.437). These results showed that resistance to
rust is controlled by a single recessive gene. These results were further corroborated with
segregation pattern in RIL population derived from the same cross. Out of 137 RILs, 78 were
susceptible and 59 were resistant and gave a good fit to the 1:1 ratio (χ 2 =2.635). The results of the
present study suggest that inheritance of rust resistance in lentil is simple and controlled by a single
recessive gene, and can be easily incorporated into locally adapted high yielding elite lines through
targeted hybridization program.
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