here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Faba bean cultures in vitro - elimination of necrotic response with antioxidant treatments
as a background for genetic transformation experiments
Helena Klenotičová, Iva Smýkalová, Lenka Švábová, Miroslav Griga
Agritec Plant Research Ltd., Šumperk, Czech Republic
Faba bean represents one of the in vitro recalcitrant legumes due to frequent deterioration of
explant material and cultivated tissues as a result of the action of phenolic compounds. Ascorbic
acid, citric acid, glutathione and active charcoal were used in a set of experiments with low-tanin
faba bean cultivars aimed to achieve efficient regeneration protocol. The application of
antioxidants led to decreased callogenesis citric acids treatments minimised necrotic response of
explants. Citric acid, used together with ascorbic acid completely inhibited shoot growth in apex
cultures. Glutathion evoked hyperhydricity of explants. Active charcoal induced rooting on media
which are commonly used for shoot proliferation. In subsequent testing, the effects of
combination with transformation co-cultivation compounds acetosyringone and L-cysteine was
studied. Combination of acetosyringone with antioxidants did not negatively influenced shoot
proliferation, except of variant with ascorbic acid. Our experimental findings recognized citric
acid, L-cysteine and L-acetosyringone as the helpful agents. Citric acid worked as the best and
universal antioxidant in faba bean in vitro cultures utilizable also for further genetic transformation
experiments.
Acknowledgements
The work is financially supported by National Agency for Agricultural Research of Ministry of Agriculture CR, grant
No. QI91A229.
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