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Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Strategies to identify candidate genes controlling Ascochyta blight in different legume crops S Ocaña-Moral 1 , E Madrid 2 , T Millán 3 , P García 4 , M Pérez de la Vega 4 , AM Torres 1 1 Área Mejora y Biotecnología, IFAPA-Centro Alameda del Obispo, Córdoba, Spain 2 Instituto de Agricultura Sostenible, CSIC, Mejora Genética Vegetal, Córdoba, Spain 3 Universidad de Córdoba, Dpto. Genética, Campus de Rabanales Edificio C5 2a planta, Córdoba, Spain Until now complete resistance to Ascochyta blight has not been reported neither in chickpea nor in faba bean. However, several QTLs have been located in chickpea LGs 2, 3, 4, 6 and 8. The most significant ones are those in LGs 2 (QTL AR3) and 4 (QTL AR1 and QTL AR2). Attempts to saturate QTL1 AR1 have been performed by developing BAC clones or by identifying candidate genes from the model Medicago truncatula. In faba bean at least two QTLs (Af1 and Af2), assigned to chromosomes III and II were validated across environments and generations. In a second cross, six QTLs (Af3 to Af8), were detected. Af1 and Af3 were both assigned to chromosome III and might correspond to the same genomic region. In this study, we are exploiting the synteny with the model species M. truncatula and chickpea, to establish a clear homology of QTL AR1 and Af1 in both crops based on ESTs mapping. Moreover, possible regulators revealed by SuperSAGE analysis and associated with the ascochyta-faba bean and ascochyta-lentil interactions are being mapped in the corresponding maps in order to identify potential targets for molecular breeding. Finally, we will take profit from the recent chickpea whole genome sequence, to perform wide genome comparative mapping studies with the less-studied legume crops, in order to identify orthologous genes underlying not only blight resistance but other agronomic important traits. Our aim is to progress from basic mapping of QTLs to fine mapping or gene identification, thus allowing development of efficient markers for MAS in breeding. 167
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Double haploids in pigeon pea: Interolog mapping of putative candidate genes Sameera Panchangam 1, 3 , Prashanth Suravajhala 2 , Nalini Mallikarjuna 1 1 International Crop Research Institute for Semi-Arid Tropics, Patancheru, India 2 Bioclues Organization, IKP Knowledge Park, Picket, Secunderabad, India 3 Jawaharlal Nehru Institute of Advanced Studies, Secunderabad, India Double haploids (DH) are promising tools for developing pure breeding lines with complete homozygosity in a single generation. Crop plants such as pigeon pea (Cajanus cajan (L.) Millsp.), which is a self-pollinated crop with a narrow genetic base, greatly benefit from DH technology by accelerating cultivar development. The key to successful production of DH is in the identification of gene networks, the expressed proteins and interacting partners. Independent studies in model crops for DH systems such as wheat, maize, tobacco, barley and rapeseed revealed genes responsible for induction of haploidy under stress conditions. With this background, an in silico approach was employed to study the genes related to induction of haploidy in pigeonpea. Interolog based protein interaction analyses was done to identify putative candidates for these processes. Functional annotation of all proteins when searched against Arabidopsis thaliana protein sequences showed few plausible proteinswhich could serve as markers and also predict the androgenic and/or DH competence of the crops of interest. 168
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First Legume Society Conference 201
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Scientific Committee Michael Abbert
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Programme 9 Session 1 Achievements
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