here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Identification and characterization of cDNA genes FLK and FVE of the autonomous
pathway of flowering Lupinus luteus
Mariusz Banach 1 , Waldemar Wojciechowski 1 , Jacek Kęsy 1 , Paulina Glazińska 1 , Emilia
Wilmowicz 1 , Agata Kućko 1 , Katarzyna Marciniak 1 , Jan Kopcewicz 1 , Andrzej Tretyn 1,2
1
Chair of Plant Physiology and Biotechnology, Faculty of Biology and Environment Protection, Nicolaus
Copernicus University, Toruń, Poland
2
Centre for modern interdisciplinary technologies, Nicolaus Copernicus University, Toruń, Poland
The flowering time is one of the factors providing reproductive success. Four genetically defined
pathways have been identified that control flowering: the vernalization pathway, the photoperiod
pathway, the gibberellin or hormonal pathway and the autonomous pathway. All pathways
control of flowering cooperate regulation of key flowering genes which name ‘integrator genes’
or ‘central floral pathway integrators’. The autonomous pathway of flowering induction genes
includes 7 genes. All these genes are negative regulators of FLOWERING LOCUS C (FLC) -
main inhibitor of generative development. In this study, cDNA fragments FLOWERING
LOCUS KH DOMAIN (FLK) and HISTONE-BINDING PROTEIN FVE (FVE) homologous
genes were identified in Lupinus luteus genome. The sequences of these genes from other legumes
species were searched in NCBI’s database. DNA fragments of Lupinus luteus FLK (LlFLK) and
FVE (LlFVE) genes were amplified in PCR on the cDNA template used degenerate primers.
The predicted amino acid sequences were used to search conserved domain with BlastP program.
One conserved RNA-binding domain – KH motif was found in LlFLK which confirmed it is the
homolog for FLK in another plants. One conserved domain WD40 was found in LlFVE protein
which confirmed it is the homolog for FVE in another plants. Identified cDNA fragments from
L. luteus were the homolog for FLK and FVE in another plants as also confirmed by sequenced
nucleotide and predicted amino acid sequences of these genes show high homology to known
plants sequences of genes and proteins.
Acknowledgements
This research was supported by the Ministry of Agriculture and Rural Development (Poland) grants No 149/2011
and the National Science Centre (Poland) grants No 2011/01/B/NZ9/03819
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