here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
_________________________________________________________________________________________
Cross-species transferability of microsatellite markers generated from cowpea enhances
marker repertoire and genetic diversity study in Vigna genus
KP Viswanatha 1 , Poonam Singh 2 , Sidramappa Talekar 2 , DS Ambika 2 , HC Lohithaswa 2
1 University of Agricultural Sciences, Raichur, Karnataka, India
2 University of Agricultural Sciences, GKVK, Bangalore, Karnataka, India
Genus Vigna of family Leguminosae include several agriculturally important species viz., V.
unguiculata (Cowpea), V. radiata (Mungbean), V. mungo (Urdbean), V. aconitifolia (Mothbean), V.
umbellata (Ricebean) and V. angularis (Adzukibean). Except Adzuki bean, other five species are
important pulse crops in India and other parts of tropical Asia. The comparative genome analysis
to assess synteny facilitates use of genomic resources between different species which is cost
effective and can speed up gene identification in species which are less studied at the genomic
level. To study the conservation of microsatellite regions, a set of 168 cowpea specific
microsatellite markers were tested for their transferability across five species of Vigna and
horsegram. Two genotypes were chosen based on their utility in current breeding programs from
each species. In cowpea, 108 primers amplified PCR products and 47 were polymorphic between
parents of a mapping population being developed to tag genes for resistance to bacterial leaf
blight. Considerable transferability was observed with markers in Horsegram (39.9%), Ricebean
(36.4%) and Mothbean (28.6%). However, amplification rate was low in Urdbean (9.5%) and
Mungbean (9.5%). The number of polymorphic primers varied from 9 (Urdbean) to 21
(Horsegram). From the results, it is concluded that a good number of highly polymorphic
markers were identified to evaluate the genetic relationship among species of Vigna. It was also
possible to identify a comprehensive set of SSR markers as an important genomic resource for
diversity analysis and genetic mapping to test relationships of resistance to common diseases of
the members of the genus Vigna.
179