here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Identification and characterization of LlYUC cDNA involved in auxin biosynthesis in
Lupinus luteus
Jacek Kęsy 1 , Agata Kućko 1 , Emilia Wilmowicz 1 , Kamil Frankowski 1 , Katarzyna Marciniak 1 ,
Mariusz Banach 1 , Waldemar Wojciechowski 1 , Jan Kopcewicz 1 , Andrzej Tretyn 2
1 Chair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Toruń, Poland
2 Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University, Toruń, Poland
Auxin is involved either directly or indirectly in every aspect of plant development and plays the
integrative role in coordination of plant growth and development. Recently, the first complete
auxin biosynthesis pathway that converts tryptophan (Trp) to IAA has been finally established.
Initially, Trp is converted to indole-3-pyruvate (IPA) by TAA family of amino transferases and
then to IAA by YUC. This two-step conversion is the main biosynthetic pathway of auxins in
plants. In this study, YUCCA cDNA from Lupinus luteus was isolated. Coding sequence consists
of 366 bp and encodes for 121 amino acids. Comparison between the deduced amino acid
sequence of LlYUC and YUCCAs from other plant species indicates that the predicted sequence
contains characteristic Flavin-binding monooxygenase-like domain, which is essential for its
activity.
Acknowledgements
This research was supported by Ministry of Agriculture and Rural Development grant no 149/2011.
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