here - First Legume Society Conference (LSC1)

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013
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Autophagy in embryo axes of yellow lupin (Lupinus luteus L.), white lupin (Lupinus
albus L.), and Andean lupin (Lupinus mutabilis Sweet) germinating seeds
Sławomir Borek 1 , Ewelina Paluch 1 , Iwona Morkunas 2
1 Adam Mickiewicz University, Department of Plant Physiology, Poznań, Poland
2 Poznań University of Live Sciences, Department of Plant Physiology, Poznań, Poland
Experiments were conducted on embryo axes which were isolated from imbibed seeds and
culturedin vitro for 96 hon liquid mineral medium in six trophy variants: on medium with 60 mM
sucrose (+S) or without sucrose (–S) and on media additionally supplemented with asparagine
(+S+Asn and –S+Asn) or sodium nitrate (+S+NO 3 and –S+NO 3). Ultrastructural observations
were performed in embryo axes root meristematic zone cells. Sugar starvation (–S) caused a
significant increase in cell vacuolizationin cells of embryo axes of all three investigated species
(irrespectively of Asn and NO 3 nutrition).Inside vacuoles of starved cells (–S) many plasma
membrane structure were observed. Simultaneously, a decrease in phosphatidylcholine level was
observed. These results pointed on advanced autophagy.In cells of embryo axes fed with
asparagine (–S+Asn) the decomposition of autophagic bodies was remarkably lover than in cells
of non-fed with asparagine (–S) embryo axes. This allowed to more or less precise identification
of cell components which were degraded during autophagy. Inside vacuoles of –S+Asn axes the
following cell components were visible: cytoplasm, ribosomes, oil bodies, mitochondria, and
spherical objects which were looked like peroxisomes. Ultrastructure of cells of axes fed with
nitrate (–S+NO 3 and +S+NO 3) was very similar to –S and +S, respectively.
Acknowledgements
This work was supported by the National Science Centre (Grant No. NN310003540).
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