here - First Legume Society Conference (LSC1)

Recommendations

Info

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Investigating the role of solanapyrone toxins in Ascochyta blight using toxin-deficient mutants of Ascochyta rabiei W Kim 1 , HO Akamatsu 1 , TL Peever 1 , GJ Vandemark 1,2 , W Chen 1,2 1 Department of Plant Pathology, Washington State University, Pullman, USA 2 USDA-ARS, Grain Legume Genetic and Physiology Research Unit, Washington State University, Pullman, USA Ascochyta rabiei, the causal agent of Ascochyta blight of chickpea, produces solanapyrone toxins (solanapyrone A, B and C). However, very little is known about the genetics of toxin production and the role of the toxins in pathogenesis. Generating mutants deficient in the toxin biosynthesis would provide information on the role of the toxins during infection processes. Partial genomic sequences of the solanapyrone biosynthesis gene cluster in A.rabiei were identified, based on the homologous sol gene cluster (sol1 - sol6) in Alternaria solani, which also produces the same toxins. The sol5 and sol4 genes which encode solanapyrone synthase and a fungal specific transcription factor, respectively, were targeted to generate toxin-deficient mutants. Deletion of either the sol4 or the sol5 gene abolished production of solanopayrone toxins. The sol5-deletion mutants produced no solanapyrone toxxins and accumulated the precursor prosolanapyrone II, whereas thesol4-deletion mutants did not produce either solanapyrone toxins or the prosolanapyrone II. Solanopyrone toxins, but not the prosolanopyrone II precursor, showed toxicity to chickpea. Both the mutants showed normal growth patterns and conidiation in culture. Virulence of the toxin-deficient mutants was examined in pathogenicity assay using two-week old chickpea seedlings. Both sol4 and sol5 mutants showed no reduction in virulence, producing disease similar to the disease levels produced by wild-type strain. These findings indicate that sol4 gene is a positive regulator for solanapyrone biosynthesis, and that solanapyrone is not essential for the chickpea seedling infection. 203
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ The chemical communication between Bruchus rufimanus and its host-plant Vicia faba Ene Leppik, Rachid Hamidi, Brigitte Frérot The French National Institute for Agricultural Research, Versailles, France The Broad Bean Weevil (BBW) (Bruchus rufimanus Boheman) is a serious pest of the broad bean (Vicia faba Linnaeus) in Europe damaging the beans and decreasing the commercial value. Little is known on the biology and ecology of the pest. The BBW host-plant selection and colonisation was studied in France under field and laboratory conditions. In the field we observed that the BBW males arrived first during the flowering stage closely followed by the females. The colonizers were sexually mature and active BBW. Under laboratory condition, in a wind tunnel, the flowering stage of the bean revealed to significantly more attractive to females than the leave stage. The volatiles from bean plants headspace were collected at leaf, flowering stage of the bean plants to characterize the chemical signal emitted and perceived by the colonising BBW. 204
Page 2:
First Legume Society Conference 201
Page 5 and 6:
Scientific Committee Michael Abbert
Page 8:
Programme 9 Session 1 Achievements
Page 12 and 13:
Book of Abstracts First Legume Soci
Page 14:
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Page 138:
Session 6 Translational omics for l
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154: Book of Abstracts First Legume Soci
Page 226: Session 8 Non-food, non-feed and ot
Page 254 and 255:
Page 256 and 257:
Page 258 and 259:
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274:
Page 278 and 279:
Page 280 and 281:
Page 282 and 283:
Page 284 and 285:
Page 286 and 287:
Page 288:
Page 292 and 293:
Page 294 and 295:
Page 296 and 297:
Page 298 and 299:
Page 300 and 301:
Page 302 and 303:
Page 304:
Page 308 and 309:
Page 310 and 311:
Page 312:
Page 315 and 316:
Name Page Name Page Capel J 182 Duc
Page 317 and 318:
Name Page Name Page Klenotičová H
Page 319 and 320:
Name Page Name Page Prusiński J 25
Page 322 and 323:
What is IFVCNS? PLATINUM SPONSOR In
Page 324 and 325:
Agromarket www.agromarket.rs Agroma
Page 326 and 327:
CID Bio-Science, Inc. www.cid-inc.c
Page 328 and 329:
Saatzucht Steinach GmbH & Co KG Don
show all

here - First Legume Society Conference (LSC1)

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?