here - First Legume Society Conference (LSC1)

Recommendations

Info

Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Effect of endogenous signals on regulation mechanism of synthesis and accumulation of flavonoids in yellow lupine infected by Fusarium oxysporum and its impact on the cytoskeleton of plant cells Iwona Morkunas 1 , Magda Formela 1 , Jolanta Floryszak-Wieczorek 1 , Łukasz Marczak 2 , Dorota NaroŜna 3 , Witold Nowak 4 , Sławomir Borek 5 , Maciej Stobiecki 2 1 Department of Plant Physiology, Poznań University of Life Sciences, Poznań, Poland 2 Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland 3 Department of Biochemistry and Biotechnology, Poznań University of Life Sciences, Poznań, Poland 4 Laboratory of Molecular Biology Techniques, Faculty of Biology, Adam Mickiewicz University, Poznań, Poland 5 Department of Plant Physiology, Adam Mickiewicz University, Poznań, Poland Flavonoids are an important group of secondary plant metabolites with many and diverse key functions in plants. They are known as agents of defense against pathogens, with antimicrobial and antioxidant functions. Recently, they have also been shown to function as intracellular signal molecules. The first aim of the study was to examine cross-talk interactions of sucrose and infection caused by Fusarium oxysporum f.sp. lupini on expression of phenylpropanoid pathway genes and precisely the flavonoid biosynthesis pathway. Additionally, the accumulation and cytochemical localization of end products, i.e. flavonoids, were analyzed in embryo axes cells of Lupinus luteus L. cv. Juno. Moreover, it was checked whether the accumulation of flavonoids, especially genistein affects on the changes in the cytoskeleton of cells. The second aim of the study was to examine cross-talk of exogenous nitric oxide (NO) and sucrose in the mechanisms of synthesis and accumulation of isoflavonoids in yellow lupine embryo axes. It was verified whether the dialogue of these molecules can modulate defence response of axes to infection and development of the pathogenic fungus F. oxysporum. The experimental protocol used in this study was based on a model system: embryo axes from germinating yellow lupine seeds inoculated with Fusarium oxysporum f.sp. lupini and cultured in vitro on a medium with or without sucrose, analyzed to 96 h after inoculation. The obtained results show that sucrose as signal molecule stimulated phenylpropanoid metabolism. Sucrose strongly stimulated the expression of the flavonoid biosynthetic genes, i.e. phenylalanine ammonialyase (PAL), chalcone synthase (CHS), chalcone isomerase (CHI) and isoflavone synthase (IFS). The sensing of carbohydrate levels and the response to fungal pathogens may be interrelated at some level. In the early phase of infection the flavonoid biosynthesis pathway is considerably enhanced in yellow lupine embryo axes as a strong signal amplification effect of sucrose and the pathogenic fungus F. oxysporum. Moreover the strong accumulation of isoflavonoids in inoculated F. oxysporum embryo axes of yellow lupine pretreated with a nitric oxide donor with a high level of sucrose is a result of amplification of the signal coming from sucrose, the nitric oxide donor and the pathogenic fungus. Sucrose and nitric oxide act as signal molecules, which correlated with up-regulation of genes engaged in isoflavonoids production. Acknowledgments This study was supported by the Polish Committee for Scientific Research (KBN, grant no. N N303 414437) 209
Book of Abstracts First Legume Society Conference 2013: A Legume Odyssey Novi Sad, Serbia, 9-11 May 2013 _________________________________________________________________________________________ Response of dry bean (Phaseolus vulgaris L.) genotypes to water shortage Mustafa Onder, Ali Kahraman, Ercan Ceyhan University of Selcuk, Faculty of Agriculture, Department of Field Crops, Konya, Turkey This research was conducted to determine optimum timing and frequency of common bean irrigation in Konya. Field trial was conducted according to “Randomized Complete Blocks Design” with three replications in Selcuk University, Agricultural Faculty, Campus-Konya trial fields during the year of 2009. Sowings were made on 15 th of May and harvest was made on September. After sowing, the plots were irrigated by sprinkler for only two hours to provide the emergence. A total of 41 common dry bean genotypes were grown in field conditions with only 2 drip irrigations during the flowering (50 th day after sowing) and pod filling (58 th day after sowing) periods for 6 hours (from 0am to 6am) per irrigation which was provided enough water to effective root depth. Some agronomical characteristics were determined on behalf of plant response to limited water. According to the results, number of main branch per plant was significant on the level of P
Page 2:
First Legume Society Conference 201
Page 5 and 6:
Scientific Committee Michael Abbert
Page 8:
Programme 9 Session 1 Achievements
Page 12 and 13:
Book of Abstracts First Legume Soci
Page 14:
Page 18 and 19:
Page 20 and 21:
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46:
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60 and 61:
Page 62 and 63:
Page 64 and 65:
Page 66 and 67:
Page 68:
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79:
Page 80 and 81:
Page 82 and 83:
Page 84 and 85:
Page 86 and 87:
Page 88 and 89:
Page 90 and 91:
Page 92 and 93:
Page 94 and 95:
Page 96 and 97:
Page 98 and 99:
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Page 138:
Session 6 Translational omics for l
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Page 149 and 150:
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160: Book of Abstracts First Legume Soci
Page 226: Session 8 Non-food, non-feed and ot
Page 260 and 261:
Page 262 and 263:
Page 264 and 265:
Page 266 and 267:
Page 268 and 269:
Page 270 and 271:
Page 272 and 273:
Page 274:
Page 278 and 279:
Page 280 and 281:
Page 282 and 283:
Page 284 and 285:
Page 286 and 287:
Page 288:
Page 292 and 293:
Page 294 and 295:
Page 296 and 297:
Page 298 and 299:
Page 300 and 301:
Page 302 and 303:
Page 304:
Page 308 and 309:
Page 310 and 311:
Page 312:
Page 315 and 316:
Name Page Name Page Capel J 182 Duc
Page 317 and 318:
Name Page Name Page Klenotičová H
Page 319 and 320:
Name Page Name Page Prusiński J 25
Page 322 and 323:
What is IFVCNS? PLATINUM SPONSOR In
Page 324 and 325:
Agromarket www.agromarket.rs Agroma
Page 326 and 327:
CID Bio-Science, Inc. www.cid-inc.c
Page 328 and 329:
Saatzucht Steinach GmbH & Co KG Don
show all

here - First Legume Society Conference (LSC1)

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?